Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila.
Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila.
复制标题
剖析果蝇睾丸肌管迁移的集体细胞行为
DOI：
10.1007/978-1-0716-2887-4_8
复制
发表时间：
2022
影响因子：
--
通讯作者：
S. Bogdan
中科院分区：
文献类型：
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作者：
Bischoff;S. Bogdan
集体细胞迁移在组织形态发生、伤口愈合、组织再生和癌症侵袭中起着关键作用。近年来，已经建立了不同的动物模型来分析化学和机械刺激如何塑造单个细胞的行为成为组织和器官。目前，仍然只有少数模型系统能够以高分辨率实时地从遗传学角度解剖组织形态发生过程中驱动细胞运动的潜在分子机制。在这里，我们为果蝇的离体培养提供了详细的方案和工具箱，用于肌管集体迁移的4D实时成像，这可以从遗传学上解决关于丝状足的突起/运动模式、细胞-细胞粘附、集体决策的细胞骨架模式和集体关闭过程等广泛的发育和细胞生物学问题。此外，该方案已成功地与激光诱导单细胞消融和药物治疗联合使用，但也可以在组织固定后与共聚焦显微镜一起使用。
Collective cell migration has a key role in tissue morphogenesis, wound healing, tissue regeneration, and cancer invasion. In recent years, different animal models have been established to analyze how chemical and mechanical stimuli shape the behavior of single cells into tissues and organs. At present, there are still only a few model systems that allow to genetically dissect underlying molecular mechanisms driving cell motility during tissue morphogenesis at high resolution in real time. Here, we provide a detailed protocol and toolbox for ex vivo culturing ofDrosophilatestes for 4D live imaging of myotube collective migration, which allows to genetically address a wide range of developmental and cell biological questions regarding modes of filopodia-based protrusion/locomotion, cell-cell adhesion, cytoskeletal modes of collective decision-making, and collective closure processes. Additionally, this protocol has been successfully used in combination with laser-induced single-cell ablation and pharmacological treatments, but it can also be used with confocal microscopy after tissue fixation.