Correlation Between DNA Methylation of TRPA1 and Chronic Pain States in Human Whole Blood Cells.
Correlation Between DNA Methylation of TRPA1 and Chronic Pain States in Human Whole Blood Cells.
复制标题
TRPA1 的 DNA 甲基化与人类全血细胞慢性疼痛状态之间的相关性。
DOI：
10.1093/pm/pnv088
复制
发表时间：
2016
期刊：
影响因子：
3.1
通讯作者：
Munetaka Hirose
中科院分区：
文献类型：
--
作者：
Norihiko Sukenaga;Yasuko Ikeda;Daisuke Tanada;Takashi Tunetoh;Susumu Nakano;Takae Inui;Kazumi Satoh;H. Okutani;K. Noguchi;Munetaka Hirose
目标
神经免疫与外周血细胞功能变化的相互作用，包括瞬时受体电位锚蛋白1（TRPA 1）的变化，似乎在人类慢性疼痛的发展中起着关键作用。本研究的目的是研究慢性疼痛患者全血细胞中TRPA1 DNA甲基化与疼痛状态之间的关系。
方法
在收集了12名慢性疼痛患者的血液样本后，作者测量了全血细胞中的DNA甲基化水平。患者的人口统计学数据和慢性疼痛状态之间的显著关联通过多元线性回归分析来确定，该分析使用年龄、体重指数、疼痛持续时间、抑郁、焦虑、认知障碍、日常生活活动、神经性疼痛和疼痛状态作为因变量，TRPA 1 DNA甲基化水平作为自变量。
结果
多元回归分析显示，TRPA1基因中CpG岛甲基化水平的增加与神经性疼痛症状数量的增加之间存在显著相关性，这些症状使用Doubletree Neuropathique 4（DN4）问卷进行评估。TRPA1 mRNA表达的降低也与DN4评分的增加显著相关。烧灼感的存在，这是DN4问卷中的疼痛症状之一，与TRPA1的DNA甲基化水平的增加显著相关。
结论
全血细胞中TRPA1 DNA甲基化水平似乎与慢性疼痛患者的疼痛症状相关。
OBJECTIVES
Neuro-immune interactions with functional changes in the peripheral blood cells including changes in the transient receptor potential ankyrin 1 (TRPA1) appear to play a pivotal role in the development of chronic pain in humans. The aim of this study was to examine the association between TRPA1 DNA methylation in whole blood cells and the pain states in chronic pain patients.
METHODS
After collecting blood samples from 12 chronic pain patients, the authors measured DNA methylation levels in whole blood cells. Significant associations between the patient's demographic data and the chronic pain states were determined by a multiple linear regression analysis that used age, body mass index, pain duration, depression, anxiety, cognitive impairment, activities of daily living, neuropathic pain, and pain states as the dependent variables, and the TRPA1 DNA methylation levels as the independent variables.
RESULTS
Multiple regression analysis revealed a significant correlation between increases of the methylation levels of the CpG island in the TRPA1 gene and increases in the number of neuropathic pain symptoms, which were evaluated using the Douleur Neuropathique 4 (DN4) questionnaire. Decreases in the TRPA1 mRNA expression were also significantly related to increases in the DN4 score. The presence of a burning sensation, which is one of pain symptoms in the DN4 questionnaire, was significantly correlated with the increase in DNA methylation level of TRPA1.
CONCLUSIONS
TRPA1 DNA methylation levels in whole blood cells appear to be associated with pain symptoms in chronic pain patients.
DOI：
10.1016/j.trsl.2014.05.012
发表时间：
2015-01
期刊：
Translational research : the journal of laboratory and clinical medicine
影响因子：
--
作者：
Bai G;Ren K;Dubner R
通讯作者：
Dubner R