HUMAN MALARIA PARASITES IN CONTINUOUS CULTURE
HUMAN MALARIA PARASITES IN CONTINUOUS CULTURE
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DOI：
10.1126/science.781840
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发表时间：
1976-01-01
期刊：
影响因子：
56.9
通讯作者：
JENSEN, JB
中科院分区：
文献类型：
--
作者：
TRAGER, W;JENSEN, JB
恶性疟原虫可以在38 ℃孵育的人红细胞中连续培养。C在含有人血清的RPMI 1640培养基中，在具有7%CO2和低O2（1或5%）的气氛下。通过以3或4天的间隔加入人红细胞，将来自感染的Aotus trivirgatus猴的原始寄生虫材料稀释> 1亿倍。寄生虫继续在其正常的无性生殖周期约48小时，但不再高度同步。它们仍然对Aotus有感染力。
Plasmodium falciparum can be maintained in continuous culture in human erythrocytes incubated at 38.degree. C in RPMI 1640 medium with human serum under an atmosphere with 7% CO2 and low O2 (1 or 5%). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted > 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of about 48 h but were no longer highly synchronous. They remained infective to Aotus.