Common gene expression strategies revealed by genome-wide analysis in yeast
Common gene expression strategies revealed by genome-wide analysis in yeast
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DOI：
10.1186/gb-2007-8-10-r222
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发表时间：
2007-01-01
期刊：
影响因子：
12.3
通讯作者：
Perez-Ortin, Jose E.
中科院分区：
文献类型：
--
作者：
Garcia-Martinez, Jose;Gonzalez-Candelas, Fernando;Perez-Ortin, Jose E.
背景：基因表达是一个两步合成的过程，以执行其功能所需的每种蛋白质的必要量结束。由于蛋白质是最终产物，因此基因调控的主要焦点应该是蛋白质。然而，由于mRNA是一个中间步骤，mRNA和蛋白质的量都由它们的合成和降解速率控制，因此可以通过不同的策略来实现所需的蛋白质量。在本文中，我们首次全面分析了生物体中表征基因表达的六个变量之间的关系：转录和翻译速率、mRNA和蛋白质量以及mRNA和蛋白质稳定性。我们使用了以前发表的数据，从指数增长的酿酒酵母细胞。我们发现，有一个普遍的趋势，协调mRNA和蛋白质的水平，通过协调其合成率和功能相关的基因往往有类似的值为6个variable.Conclusion：我们建议，酵母细胞使用共同的表达策略，基因在相同的生理途径。这种趋势在编码大而稳定的蛋白质复合物（如核糖体或蛋白酶体）的基因中更为明显。因此，每个功能组可以被定义为一个“六个变量的配置文件”，说明了共同的策略，然后由包括在它的基因。基因编码亚基的蛋白质复合物显示出一种倾向，有相对不稳定的mRNA和一个不太平衡的配置文件的mRNA比蛋白质，这表明在转录水平上更强的调节。
Background: Gene expression is a two-step synthesis process that ends with the necessary amount of each protein required to perform its function. Since the protein is the final product, the main focus of gene regulation should be centered on it. However, because mRNA is an intermediate step and the amounts of both mRNA and protein are controlled by their synthesis and degradation rates, the desired amount of protein can be achieved following different strategies.Results: In this paper we present the first comprehensive analysis of the relationships among the six variables that characterize gene expression in a living organism: transcription and translation rates, mRNA and protein amounts, and mRNA and protein stabilities. We have used previously published data from exponentially growing Saccharomyces cerevisiae cells. We show that there is a general tendency to harmonize the levels of mRNA and protein by coordinating their synthesis rates and that functionally related genes tend to have similar values for the six variables.Conclusion: We propose that yeast cells use common expression strategies for genes acting in the same physiological pathways. This trend is more evident for genes coding for large and stable protein complexes, such as ribosomes or the proteasome. Hence, each functional group can be defined by a ' six variable profile' that illustrates the common strategy followed by the genes included in it. Genes encoding subunits of protein complexes show a tendency to have relatively unstable mRNAs and a less balanced profile for mRNA than for protein, suggesting a stronger regulation at the transcriptional level.