Microfluidics assisted generation of innovative polysaccharide hydrogel microparticles
Microfluidics assisted generation of innovative polysaccharide hydrogel microparticles
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DOI：
10.1016/j.carbpol.2014.01.083
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发表时间：
2015-02-13
影响因子：
11.2
通讯作者：
Renard, D.
中科院分区：
文献类型：
--
作者：
Marquis, M.;Davy, J.;Renard, D.
基于毛细管流动的方法如微流体装置提供了许多优于常规流动控制技术的优点，因为它们确保了高度通用的几何形状，并且可以用于生产单分散的球形和非球形聚合物微粒。基于流动聚焦装置在有机相中使水溶液共流的原理，我们能够生产以下创新的多糖水凝胶微粒：生产由果胶-果胶（homo Janus）和果胶-藻酸盐（hetero Janus）制成的Janus水凝胶微粒。通过共聚焦扫描激光显微镜（CSLM）的先前标记的生物聚合物的两个半球的分离的效率进行了研究。通过使每个微粒半球经受特定酶促降解来确认Janus结构。作为概念证明，游离BSA或接枝有葡聚糖的BSA包封在异Janus水凝胶微粒的每个半球中。而BSA，游离或接枝葡聚糖，总是局限在藻酸盐半球，BSA的一部分从果胶扩散到藻酸盐半球。果胶链上沿着的甲氧基基团将减少BSA的氨基与果胶的羧基之间发生的吸引静电相互作用的数量。以碳酸二甲酯为连续相，结合凝胶化和水扩散诱导的自组装现象，在芯片上成功制备了复杂形状的果胶水凝胶微粒，或通过在流体-流体界面处芯片外的预胶凝液滴的变形。球形，扁椭圆形，环面或蘑菇型的形态，从而获得。此外，它被确定为在收集过程中穿过界面后，蘑菇型微粒不迁移在钙或DMC相，但留在液-液界面。这些新的和原始的水凝胶微粒将打开机会，研究结合酶水解和活性释放的Janus颗粒和形状和溶胀行为之间的关系，各向异性果胶微粒。(C)2014爱思唯尔有限公司版权所有。
Capillary flow-based approach such as microfluidic devices offer a number of advantages over conventional flow control technology because they ensure highly versatile geometry and can be used to produce monodisperse spherical and non-spherical polymeric microparticles. Based on the principle of a flow-focusing device to emulsify the coflow of aqueous solutions in an organic phase, we were able to produce the following innovative polysaccharide hydrogel microparticles:Janus hydrogel microparticles made of pectin-pectin (homo Janus) and pectin-alginate (hetero Janus) were produced. The efficiency of separation of the two hemispheres was investigated by confocal scanning laser microscopy (CSLM) of previously labelled biopolymers. The Janus structure was confirmed by subjecting each microparticle hemisphere to specific enzymatic degradation. As a proof of concept, free BSA or BSA grafted with dextran, were encapsulated in each hemisphere of the hetero Janus hydrogel microparticles. While BSA, free or grafted with dextran, was always confined in the alginate hemisphere, a fraction of BSA diffused from the pectin to the alginate hemisphere. Methoxy groups along the pectin chain will be responsible of the decrease of the number of attractive electrostatic interactions occurring between amino groups of BSA and carboxylic groups of pectin.Pectin hydrogel microparticles of complex shapes were successfully produced by combining on-chip the phenomenon of gelation and water diffusion induced self-assembly, using dimethyl carbonate as continuous phase, or by deformation of the pre-gelled droplets off-chip at a fluid-fluid interface. Sphere, oblate ellipsoid, torus or mushroom-type morphologies were thus obtained. Moreover, it was established that after crossing the interface during their collect, mushroom-type microparticles did not migrate in the calcium or DMC phase but stayed at the liquid-liquid interface.These new and original hydrogel microparticles will open up opportunities for studying relationships between combined enzymatic hydrolysis and active release for Janus particles and relationships between shape and swelling behaviour for anisotropic pectin microparticles. (C) 2014 Elsevier Ltd. All rights reserved.