In Vivo Quantitative Evaluation of the Rat Retinal Nerve Fiber Layer with Optical Coherence Tomography
In Vivo Quantitative Evaluation of the Rat Retinal Nerve Fiber Layer with Optical Coherence Tomography
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DOI：
10.1167/iovs.08-2764
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发表时间：
2009-06-01
影响因子：
4.4
通讯作者：
Sugiyama, Kazuhisa
中科院分区：
文献类型：
--
作者：
Nagata, Atsushi;Higashide, Tomomi;Sugiyama, Kazuhisa
目的。确定光学相干断层扫描（OCT）是否可用于定量评估视神经挤压模型中大鼠视网膜神经纤维层（RNFL）的厚度。方法。采用改进的商用时域 OCT 和带宽为 150 nm 的超发光二极管开发了 OCT 系统。优化光学组件以获取大鼠视网膜图像。右视神经被夹子在眶内夹碎。左眼作为未经治疗的对照。在挤压前和挤压后1、2、4周进行以视盘为中心的直径为500μm的环视乳头OCT扫描。对 RNFL 厚度测量的重复性和再现性进行了评估。将通过线性垂直 OCT 扫描确定的距视盘中心 400、500 和 600 μm 处的 RNFL 厚度与视网膜切片的厚度进行比较。挤压前和挤压后 1、2 和 4 周，乳头周围 OCT 扫描中的平均 RNFL 厚度分别为 27.9 +/- 1.8、29.2 +/- 2.4、19.9 +/- 2.3 和 4.5 +/- 3.6 μm。 RNFL 厚度在挤压后 1 周没有变化，但在第二周后显着且逐渐下降（P < 0.01）。除了 4 周时压伤的眼睛外，重复性和再现性系数均小于 10%。 OCT 图像中的 RNFL 厚度与组织学测定的厚度显着相关（r = 0.90，P < 0.001）。结论。 OCT 是定量评估大鼠 RNFL 厚度的有用且有价值的工具。 （投资眼科可见科学。2009 年；50：2809-2815）DOI：10.1167/iovs.08-2764
PURPOSE. To determine whether optical coherence tomography (OCT) is useful for quantitative evaluation of the thickness of the rat retinal nerve fiber layer (RNFL) in an optic nerve crush model.METHODS. An OCT system was developed with a modified commercial time-domain OCT and a superluminescent diode with a bandwidth of 150 nm. Optical components were optimized to acquire rat retinal images. The right optic nerve was crushed intraorbitally with a clip. The left eye served as the untreated control. Circumpapillary OCT scans with a circle diameter of 500 mu m centered on the optic disc were performed before and 1, 2, and 4 weeks after the crush. Repeatability and reproducibility of RNFL thickness measurements were evaluated. The RNFL thicknesses at 400, 500, and 600 mu m from the center of the optic disc determined by linear vertical OCT scans were compared with thicknesses in retinal sections.RESULTS. The mean RNFL thicknesses in circumpapillary OCT scans were 27.9 +/- 1.8, 29.2 +/- 2.4, 19.9 +/- 2.3, and 4.5 +/- 3.6 mu m before and 1, 2, and 4 weeks after the crush, respectively. RNFL thickness was unchanged 1 week after the crush, but then decreased significantly and progressively after the second week (P < 0.01). Coefficients of repeatability and reproducibility were less than 10% except for the crushed eyes at 4 weeks. RNFL thicknesses in OCT images correlated significantly with thicknesses determined histologically (r = 0.90, P < 0.001).CONCLUSIONS. OCT is a useful and valuable tool for quantitative evaluation of rat RNFL thickness. (Invest Ophthalmol Vis Sci. 2009; 50: 2809-2815) DOI: 10.1167/iovs.08-2764