Effect of propofol on cardiac function and gene expression after ischemic-reperfusion in isolated rat heart.
Effect of propofol on cardiac function and gene expression after ischemic-reperfusion in isolated rat heart.
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DOI：
10.4097/kjae.2010.58.2.153
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发表时间：
2010-02
影响因子：
2.9
通讯作者：
Choi SU
中科院分区：
文献类型：
--
作者：
Kim YJ;Lim HJ;Choi SU
本研究的目的是检查缺血再灌注后心脏功能和心脏对异丙酚的转录反应。使用改良的 Krebs-Henseleit 缓冲液对大鼠心脏进行 Langendorff 灌注，并经历 20 分钟的稳定期、40 分钟的缺血期和 120 分钟的再灌注期。心脏被分为5组；对照：稳定后180分钟灌注，缺血：稳定后40分钟整体缺血，然后再灌注120分钟，前：仅在缺血前进行2μM异丙酚治疗，后：仅在缺血后再灌注期间进行2μM异丙酚治疗，前/后：在缺血之前和之后均进行2μM异丙酚治疗。获得心脏性能的测量结果，例如左心室展开压（LVDP）、左心室压力产生率（dP/dt）、心率和冠状动脉流量。使用安捷伦微阵列测定分离的 mRNA 的表达谱，并使用实时聚合酶链式反应 (RT-PCR) 来确认基因子集的微阵列结果。术后组显示出比缺血组更好的 LVDP 和 dP/dt。但各组之间的心率和冠脉流量没有显着差异。 RT-PCR结果显示，与缺血组相比，Post组Abcc9、Bard1和Casp4的表达增加，而Lyz、Casp8和Timp1的表达减少。这项研究表明，2 µM 异丙酚可能在离体大鼠心脏再灌注过程中提供心脏保护作用，并调节细胞凋亡和 KATP 离子通道相关基因等基因表达。
The aim of this study was to examine the cardiac function and transcriptional response of the heart to propofol after ischemia-reperfusion. Rat hearts were Langendorff-perfused using the modified Krebs-Henseleit buffer, and took 20 min stabilizing periods, 40 min ischemia periods, and then 120 min reperfusion period. The hearts were divided into 5 groups; Control: 180 min perfusion after stabilization, Ischemic: 40 min global ischemia after stabilization, followed by 120 min reperfusion, Pre: 2 µM propofol treatment was preformed only before ischemia, Post: 2 µM propofol treatment was performed only during reperfusion after ischemia, Pre/Post: 2 µM propofol treatment was performed both before and after ischemia. The measurement for cardiac performances, such as left ventricular developed pressure (LVDP), rate of left ventricular pressure generation (dP/dt), heart rate, and coronary flow were obtained. The expression profiles of isolated mRNA were determined by using Agilent microarray and real time-polymerase chain reaction (RT-PCR) was used to confirm the microarray results for a subset of genes. The Post group showed better LVDP and dP/dt than the Ischemic group. But there were no significant differences in heart rate and coronary flow among the groups. On the results of RT-PCR, the expressions of Abcc9, Bard1, and Casp4 were increased, but the expressions of Lyz, Casp8, and Timp1 were decreased in the Post group compared with the Ischemic group. This study suggests that 2 µM propofol may provide cardioprotective effect, and modulate gene expression such as apoptosis, and KATP ion channel related-genes during reperfusion in the isolated rat hearts.