Quantitative analysis of the dendrites of sacral preganglionic neurons in the cat.
Quantitative analysis of the dendrites of sacral preganglionic neurons in the cat.
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猫骶神经节前神经元树突的定量分析。
DOI：
10.1002/cne.1269
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Ohara,PT
中科院分区：
文献类型：
--
作者：
Morgan,CW;Ohara,PT
用神经生物素或辣根过氧化物酶细胞内注射方法，对25个经电生理鉴定的猫脊髓节前神经元(PGN)的树突和胞体进行了定量分析。测量了每个枝晶的总枝晶长度和表面积。树干树枝根部的大小与全树的大小和端枝的数量呈正相关。体细胞和树突的总表面积平均为39,138μm2，其中90.7%来自树突。为了测量PGN树突对脊髓特定区域的相对贡献，记录了每个树突占据8个脊髓区域的百分比。63%的树突投射到躯体的背侧，而平均33.3%的树突位于白质，其中大部分位于外侧和背外侧穹隆。这个样本中的神经元形成了一个连续体，其中一些神经元在I层有很大比例的树突，但在白质中很少，而在连续体的另一端是相反构型的细胞。中间神经元在两个部位都有树突。综上所述，这些数据表明，在骶骨副交感神经核侧带内有一个异质性的PGN群体。J.Comp.神经。437：56-69,2001。©2001 Wiley-Liss公司
Quantitative analyses were performed on the dendrites and somata of 25 electrophysiologically identified preganglionic neurons (PGN) obtained from the sacral spinal cord of the cat by intracellular injection of Neurobiotin or horseradish peroxidase. Total dendritic length and surface area were measured for each dendrite. The sizes of the stem dendrites measured at their base were positively correlated with the sizes of the entire tree and numbers of end branches. Total surface area of somata and dendrites averaged 39,138 μm2; 90.7% of that was from the dendrites. To obtain measurements of the relative contributions of PGN dendrites to specific regions of the spinal cord, the percentage of each dendrite occupying eight spinal cord regions was recorded. Sixty‐three percent of the dendrites projected dorsal to their somata, whereas an average of 33.3% of dendrites were located in the white matter, most of them in the lateral and dorsolateral funiculi. The neurons within this sample formed a continuum with some neurons having a large percentage of dendrites in lamina I but little in the white matter, whereas at the other end of the continuum were cells with the reverse configuration. The intermediate neurons had dendrites in both locations. Taken together, these data indicate a heterogeneous population of PGN in the lateral band of the sacral parasympathetic nucleus. J. Comp. Neurol. 437:56–69, 2001. © 2001 Wiley‐Liss, Inc.