Salivary Proteins of Lygus hesperus (Hemiptera: Miridae)
Salivary Proteins of Lygus hesperus (Hemiptera: Miridae)
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DOI：
10.1603/an12096
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发表时间：
2013-01-01
影响因子：
2.3
通讯作者：
Puterka, Gary J.
中科院分区：
文献类型：
--
作者：
Cooper, W. Rodney;Nicholson, Scott J.;Puterka, Gary J.
美洲斑潜蝇Lygus heperus Knight(半翅目：斑潜蝇科)对美国西部地区的多种农作物造成经济损失。在刺刺的过程中，橙皮鱼会分泌唾液，唾液中含有裂解酶，可促进宿主组织的口外消化。猕猴桃唾液主要由多聚半乳糖醛酸酶、α-淀粉酶和蛋白水解酶组成，但唾液中除这些酶外的其他成分尚未见文献记载。提高对猕猴桃唾液蛋白的认识有助于更好地了解寄主与寄主之间的相互作用，并有助于培育出能抵抗或耐受寄主毒害的作物。本研究的目的是利用质谱学方法鉴定柑橘成虫唾液蛋白。多聚半乳糖醛酸酶和多聚半乳糖醛酸酶从刺针探查和摄食的饲料中鉴定出来。用质谱仪鉴定了刺参饲料中的漆酶、烷基过氧化氢还原酶类蛋白、葡萄糖脱氢酶和黄嘌呤脱氢酶，并用比色法测定了漆酶活性。这是首次从猕猴桃唾液中鉴定出漆酶、葡萄糖脱氢酶和黄嘌呤脱氢酶；我们提出了这些酶作为植物防御化合物的靶标。细菌植物病原菌Pantoea ananatis的近100个蛋白质和沙雷氏菌的9个蛋白质。在刺针探查的饮食中被发现。这些结果表明，麻黄杆菌可能携带这些植物病原菌。我们的研究增加了已记录的猕猴桃唾液蛋白的列表，并为进一步研究猕猴桃唾液和狼疮与微生物的相互作用提供了有用的信息。
Lygus hesperus Knight (Hemiptera: Miridae) causes economic damage to a wide range of crops in the western United States. While stylet-probing, L. hesperus discharge saliva consisting of lytic enzymes that facilitate extraoral digestion of host tissues. L. hesperus saliva primarily consists of polygalacturonases, alpha-amylases, and proteases, but salivary constituents other than these enzymes have not been documented. Improved knowledge of L. hesperus salivary proteins could lead to a better understanding of Lygus-host interactions, and aid in the development of crops that are resistant or tolerant to injury caused by Lygus spp. The objective of our study was to use mass spectrometry to identify salivary proteins of L. hesperus adults. Multiple polygalacturonases and proteases were identified from diet that was stylet-probed and fed upon by L. hesperus. Laccase, alkyl hydroperoxide reductase-like protein, glucose dehydrogenase, and xanthine dehydrogenase were identified in stylet-probed diets by using mass spectrometry, and laccase activity was confirmed using a colorimetric assay. This is the first study to identify laccase, glucose dehydrogenase, and xanthine dehydrogenase from L. hesperus saliva; we propose these enzymes target plant-defense compounds. Nearly 100 proteins from the bacterial plant pathogen, Pantoea ananatis and nine proteins from Serratia spp. were identified in stylet-probed diets. These results suggest L. hesperus may vector these plant pathogens. Our study adds to the list of documented L. hesperus salivary proteins, and provides information that will be useful for the further study of L. hesperus saliva and Lygus-microbe interactions.