Efficient gene transfer into murine pancreatic islets using adenovirus vectors
Efficient gene transfer into murine pancreatic islets using adenovirus vectors
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DOI：
10.1016/j.jconrel.2007.01.012
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发表时间：
2007-05-14
影响因子：
10.8
通讯作者：
Mizuguchi, Hiroyuki
中科院分区：
文献类型：
--
作者：
Mukai, Eri;Fujimoto, Shimpei;Mizuguchi, Hiroyuki
我们利用腺病毒（Ad）载体研究了基因转导到小鼠胰岛的效率。Western blotting分析显示，小鼠胰岛表达柯萨奇病毒和腺病毒受体（一种Ad受体）。然而，在体外，Ad载体转导后的基因表达仅在胰岛周围观察到，这可能是由于对胰岛内部细胞感染Ad的物理阻碍。在Ad载体转导前无Ca2+处理提高了胰岛的转导效率，但对胰岛内部的细胞没有作用。Ad载体在体内通过腹腔动脉进行转导，然后在体外培养胰岛，即使在胰岛内部也能进行有效的转导。因此，我们提出了一种有效的基因转移到胰腺β细胞的新策略。(c) 2007 Elsevier b.v.版权所有
We investigated the efficiency of gene transduction into murine pancreatic islets using the adenovirus (Ad) vector. Western blotting analysis showed that mouse pancreatic islets express coxsackievirus and adenovirus receptor, a receptor for Ad. Nevertheless, gene expression after transduction of the Ad vector in vitro was observed only in the periphery of the islets, probably due to physical obstruction against Ad infection of the cells in the inside of islets. Ca2+-free treatment before the Ad vector transduction enhanced transduction efficiency in the islets, but not the cells in the inside of islets. The Ad vector transduction through the celiac artery in vivo and then cultivation of islets in vitro resulted in efficient transduction even in the inside of islets. Thus we propose a new strategy for efficient gene transfer to pancreatic beta-cells. (c) 2007 Elsevier B.V All rights reserved.