IL-23R Deficiency Does Not Impact Atherosclerotic Plaque Development in Mice.
IL-23R Deficiency Does Not Impact Atherosclerotic Plaque Development in Mice.
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DOI：
10.1161/jaha.117.008257
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发表时间：
2018-04-04
影响因子：
5.4
通讯作者：
Lichtman AH
中科院分区：
文献类型：
--
作者：
Engelbertsen D;Depuydt MAC;Verwilligen RAF;Rattik S;Levinsohn E;Edsfeldt A;Kuperwaser F;Jarolim P;Lichtman AH
Interleukin‐23 (IL‐23) has been implicated in inflammatory and autoimmune diseases by skewing CD4+ T helper cells towards a pathogenic Th17 phenotype. In this study we investigated the presence of IL‐23 receptor (IL‐23R)‐expressing cells in the atherosclerotic aorta and evaluated the effect of IL‐23R deficiency on atherosclerosis development in mice. We used heterozygous Ldlr −/− Il23r e GFP / WT knock‐in mice to identify IL‐23R‐expressing cells by flow cytometry and homozygous Ldlr −/− Il23r e GFP / eGFP (Ldlr −/− Il23r −/−) mice to investigate the effect of lack of IL‐23R in atherosclerosis. We demonstrate the presence of relatively rare IL‐23R‐expressing cells in lymphoid tissue and aorta (≈0.1–1% IL23R+ cells of all CD45+ leukocytes). After 10 weeks on a high‐fat diet, production of IL‐17, but not interferon‐γ, by CD4+ T cells and other lymphocytes was reduced in Ldlr −/− Il23r −/− compared with Ldlr −/−controls. However, Ldlr −/− and Ldlr −/− Il23r −/− mice had equivalent amounts of aortic sinus and descending aorta lesions. Adoptive transfer of IL‐23R‐deficient CD4+ T cells to lymphopenic Ldlr −/− Rag1 −/− resulted in dramatically reduced IL‐17‐producing T cells but did not reduce atherosclerosis, compared with transfer of IL‐23R‐sufficient CD4+ T cells. These data demonstrate that loss of IL‐23R does not affect development of experimental atherosclerosis in LDLr‐deficient mice, despite a role for IL‐23 in differentiation of IL‐17‐producing T cells.
