Effect of pulsed electromagnetic field stimulation on knee cartilage, subchondral and epyphiseal trabecular bone of aged Dunkin Hartley guinea pigs
Effect of pulsed electromagnetic field stimulation on knee cartilage, subchondral and epyphiseal trabecular bone of aged Dunkin Hartley guinea pigs
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DOI：
10.1016/j.biopha.2007.03.001
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发表时间：
2008-12-01
影响因子：
7.5
通讯作者：
Giardino, Roberto
中科院分区：
文献类型：
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作者：
Fini, Milena;Torricelli, Paola;Giardino, Roberto
已证实脉冲电磁场(PEMF)刺激对12月龄豚鼠膝关节骨关节炎(OA)进展具有软骨保护作用。本研究的目的是为了发现PEMFS在老年动物身上是否也能在更严重的OA病变中保持疗效。15月龄的豚鼠每天(6小时/天)接受PEMF治疗。采用改良Carlsson分级的组织学/组织化学Mankin法和软骨厚度(CT)、纤颤指数(FI)、软骨下骨厚度(SBT)和骨痂微结构(骨体积：Bv/Cv；骨小梁厚度：Tb.Th；骨小梁数量：Tb.N；骨小梁间距：Tb.SP)的组织形态计量学方法对膝关节(胫骨内、外平台、股骨内侧和股骨外侧髁)进行评价。膝关节周围骨也用双X射线骨密度仪(DXA)进行了评估。PEMF刺激显著改变了所有被检查膝关节区域的OA病变的进展。在膝关节病变最严重的部位(胫骨内侧平台)，组织化学评分(p<；0.0005)、FI(p<；0.005)、体温(p<；0.05)、Bv/TV(p<；0.0005)、Tb.Th(p<；0.0005)和Tb.N(p<；0.05)显著低于假手术组，而CT(p<；0.05)和Tb.Sp(p<；0.0005)显著高于假手术组。DXA证实，假手术组动物的骨密度显著高于对照组。即使在存在严重的骨性关节炎病变的情况下，PEMFS在减少病变进展方面也保持着显着的效果。(C)2007年爱思唯尔·马森公司。版权所有。
It has been demonstrated that pulsed electromagnetic field (PEMF) stimulation has a chondroprotective effect on osteoarthritis (OA) progression in the knee joints of the 12-month-old guinea pigs. The aim of the present study was to discover whether the therapeutic efficacy of PEMFs was maintained in older animals also in more severe OA lesions.PEMFs were administered daily (6 h/day for 6 months) to 15-month-old guinea pigs. The knee joints (medial and lateral tibial plateaus, medial and lateral femoral condyles) were evaluated by means of a histological/histochemical Mankin modified by Carlsson grading score and histomorphometric measurements of cartilage thickness (CT), fibrillation index (FI), subchondral bone thickness (SBT) and epiphyseal bone microarchitecture (bone volume: BV/CV; trabecular thickness: Tb.Th; trabecular number: Tb.N; trabecular separation: Tb.SP). Periarticular knee bone was also evaluated with dual X-ray absorptiometry (DXA).PEMF stimulation significantly changed the progression of OA lesions in all examined knee areas. In the most affected area of the knee joint (medial tibial plateau), significant lower histochemical score (p < 0.0005), FI (p < 0.005), SBT (p < 0.05), BV/TV (p < 0.0005), Tb.Th (p < 0.05) and Tb.N (p < 0.05) were observed while CT (p < 0.05) and Tb.Sp (p < 0.0005) were significantly higher than in SHAM-treated animals. DXA confirmed the significantly higher bone density in SHAM-treated animals. Even in the presence of severe OA lesions PEMFs maintained a significant efficacy in reducing lesion progression. (c) 2007 Elsevier Masson SAS. All rights reserved.