A flow-based enzyme-free biosensor fabricated using track-etched membrane electrodes: Selective and sensitive detection of uric acid-猫眼课题宝

DOI：

10.1016/j.snb.2023.133588

发表时间：

2023-02

期刊：

Sensors and Actuators B: Chemical

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

H. Mizuguchi;Soma Fujiki;Takaki Shibata;Masatsugu Oishi;Masamitsu Iiyama;T. Takayanagi;Yu-Chi Lin;Min-Hsin Yeh

采用径迹蚀刻膜电极（TEMEs），研制了一种选择性、灵敏度高的流动型无酶尿酸生物传感器。所提出的传感器系统包括一个预反应器电极分解干扰物种，如L-抗坏血酸，和一个检测器电极检测尿酸。TEME通过在径迹蚀刻膜过滤器上溅射沉积金来制备。乙炔黑（AB）修饰的Au-TEME电极对尿酸的灵敏度远高于裸Au-TEME电极。插入的2-氨基乙硫醇修饰的金TEME作为预反应器电极的AB-装饰的检测器电极的上游消除了由共存的L-抗坏血酸所造成的干扰，因为干扰物种在检测尿酸之前在预反应器电极被电解。该传感器系统被纳入流动注射分析系统，以评估传感器的性能。尿酸的检出限为0.6 μM。通过标准加入人尿样的回收试验也显示了令人满意的结果。

A flow-based, enzyme-free biosensor for the selective and sensitive determination of uric acid was developed using track-etched membrane electrodes (TEMEs). The proposed sensor system comprises a pre-reactor electrode to decompose interfering species, such asL-ascorbic acid, and a detector electrode to detect uric acid. TEMEs were prepared by sputter deposition of gold on a track-etched membrane filter. Acetylene black (AB) was introduced as an electrode catalyst by simple filtration with the TEME, and the sensitivity for uric acid using the AB-decorated Au-TEME was much greater than that with bare Au-TEME. The insertion of the 2-aminoethanethiol-modified Au-TEMEs as pre-reactor electrodes upstream of the AB-decorated detector electrode eliminated the interference caused by the co-existence ofL-ascorbic acid because the interfering species were electrolyzed at the pre-reactor electrode prior to detection of uric acid. The proposed sensor system was incorporated into a flow-injection analysis system to evaluate the sensor performance. The detection limit of uric acid was 0.6 μM. A recovery test by standard addition to human urine samples also showed satisfactory results.

A flow-based enzyme-free biosensor fabricated using track-etched membrane electrodes: Selective and sensitive detection of uric acid

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