Immunohistochemical Detection of Propionibacterium acnes in the Retinal Granulomas in Patients with Ocular Sarcoidosis
Immunohistochemical Detection of Propionibacterium acnes in the Retinal Granulomas in Patients with Ocular Sarcoidosis
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DOI：
10.1038/s41598-017-15710-0
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发表时间：
2017-11-09
影响因子：
4.6
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
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作者：
Nagata, Kenji;Eishi, Yoshinobu;Kinoshita, Shigeru
结节病的病因学尚不清楚，但分枝杆菌和痤疮丙酸杆菌被认为是结节病最重要的致病菌。为了研究痤疮丙酸杆菌是否是结节病葡萄膜炎的病原体，我们用痤疮丙酸杆菌特异性单克隆抗体（PAB抗体）通过免疫组化分析了眼部结节病患者活检视网膜中检测到的痤疮丙酸杆菌的频率。11例（12只眼）结节性葡萄膜炎患者入组本研究。8例孔源性视网膜脱离患者，2例非结节性葡萄膜炎患者，2例玻璃体视网膜淋巴瘤患者作为对照。在结节病组中，肉芽肿主要见于视网膜内层，充满CD 4+细胞和CD 68+细胞，表明Th 1免疫应答。痤疮丙酸杆菌，鉴定为与PAB抗体反应的圆形体，存在于9/11例结节病患者（82%）的10/12份样本（83%）中。这些圆形小体散在视网膜肉芽肿内，主要在视网膜内层。在对照组中，未检测到圆形体。我们的研究结果表明，痤疮丙酸杆菌可能与肉样瘤葡萄膜炎。我们推测结节样肉芽肿可能是由对痤疮丙酸杆菌的Th 1免疫反应通过血液传播到视网膜而形成的。
The etiology of sarcoidosis is still obscure; however, Mycobacteria and Propionibacterium acnes are considered the most implicated etiological agent for sarcoidosis. To investigate whether P. acnes is an etiological agent for sarcoid uveitis, we analyzed the frequency of P. acnes detected within the biopsied retinas from patients with ocular sarcoidosis by immunohistochemistry with a P. acnes-specific monoclonal antibody (PAB antibody). Eleven patients (12 eyes) with sarcoid uveitis were enrolled in this study. Eight patients with rhegmatogenous retinal detachment, two patients with non-sarcoid uveitis, and two patients with vitreoretinal lymphoma were enrolled as controls. In the sarcoidosis group, granulomas were mainly observed in the inner retinal layer filled with CD4+ cells and CD68+ cells, indicating the Th1 immune response. P. acnes, identified as round bodies that reacted with the PAB antibody, were present in 10/12 samples (83%) from 9/11 patients (82%) with sarcoidosis. These round bodies were scattered within the retinal granulomas mainly in the inner retinal layer. In the control group, no round bodies were detected. Our results suggested that P. acnes could be associated with sarcoid uveitis. We hypothesize that sarcoid granulomas may be formed by a Th1 immune response to P. acnes hematogenously transmitted to the retina.