Chemical Cytometry: Fluorescence-Based Single-Cell Analysis
Chemical Cytometry: Fluorescence-Based Single-Cell Analysis
复制标题
DOI：
10.1146/annurev.anchem.1.031207.113104
复制
发表时间：
2008-01-01
影响因子：
8
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Cohen, Daniella;Dickerson, Jane A.;Dovichi, Norman J.
细胞术分析的是单个细胞的组成。流式细胞术和图像细胞术采用基于抗体的染色来表征单个细胞中的少量成分。相比之下，化学细胞术采用一套功能强大的分析工具来表征大量成分。已经开发出工具来表征单个细胞中的核酸、蛋白质和代谢物。核酸分析采用强大的基于聚合酶链反应的扩增技术，而蛋白质和代谢物分析则倾向于采用毛细管电泳分离和超灵敏的激光诱导荧光检测。现在可以在单个细胞中检测许多分析物的胞量。
Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ, antibody-based stains to characterize a handful of components in single cells. Chemical cytometry in contrast, employs a suite of powerful analytical tools to characterize a large number of components. Tools have been developed to characterize nucleic acids, proteins, and metabolites in single cells. Whereas nucleic acid analysis employs powerful polymerase chain reaction-based amplification techniques, protein and metabolite analysis tends to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection. It is now possible to detect yoctomole amounts of many analytes in single cells.