A global alfalfa diversity panel reveals genomic selection signatures in Chinese varieties and genomic associations with root development
A global alfalfa diversity panel reveals genomic selection signatures in Chinese varieties and genomic associations with root development
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DOI：
10.1111/jipb.13172
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发表时间：
2021-10-01
影响因子：
11.4
通讯作者：
Yang, Qingchuan
中科院分区：
文献类型：
--
作者：
Chen, Lin;He, Fei;Yang, Qingchuan
苜蓿（Medicago sativa L.）是世界范围内重要的饲料作物。然而，人们对育种状况和不同地理种群对苜蓿改良的影响知之甚少。在这里，我们对220份苜蓿核心种质进行了测序，并通过不同亚类之间F-ST值的比较确定中国苜蓿品种形成了一个独立的类群，这表明地理起源在类群分化中发挥着重要作用。通过追踪地理区域对中国苜蓿品种遗传多样性的影响，我们确定了350个共同候选遗传区域和686个待选基因。我们还通过全基因组关联研究定义了与 24 个重要性状相关的 165 个基因座。其中，与给定表型密切相关的 17 个基因组区域正在选择中，潜在的单倍型显示出不同地理起源的亚组之间存在显着差异。基于表达分析和关联图谱的结果，我们提出 6-磷酸葡萄糖酸内酯酶 (MsPGL) 和编码具有 NHL 结构域的蛋白质 (MsNHL) 的基因是根生长的关键候选基因。总之，我们的结果为通过分子育种改良苜蓿提供了有价值的信息。
Alfalfa (Medicago sativa L.) is an important forage crop worldwide. However, little is known about the effects of breeding status and different geographical populations on alfalfa improvement. Here, we sequenced 220 alfalfa core germplasms and determined that Chinese alfalfa cultivars form an independent group, as evidenced by comparisons of F-ST values between different subgroups, suggesting that geographical origin plays an important role in group differentiation. By tracing the influence of geographical regions on the genetic diversity of alfalfa varieties in China, we identified 350 common candidate genetic regions and 686 genes under selection. We also defined 165 loci associated with 24 important traits from genome-wide association studies. Of those, 17 genomic regions closely associated with a given phenotype were under selection, with the underlying haplotypes showing significant differences between subgroups of distinct geographical origins. Based on results from expression analysis and association mapping, we propose that 6-phosphogluconolactonase (MsPGL) and a gene encoding a protein with NHL domains (MsNHL) are critical candidate genes for root growth. In conclusion, our results provide valuable information for alfalfa improvement via molecular breeding.