miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes.
miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes.
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DOI：
10.3390/ijms17122047
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发表时间：
2016-12-07
影响因子：
5.6
通讯作者：
Yang GS
中科院分区：
文献类型：
--
作者：
Peng Y;Chen FF;Ge J;Zhu JY;Shi XE;Li X;Yu TY;Chu GY;Yang GS
MicroRNAs （miRNAs）是脂肪形成的重要调控分子。它们有助于控制前脂肪细胞的增殖和分化。先前的研究揭示了miR-429在细胞侵袭、迁移和凋亡中的重要作用。我们之前的工作表明，在新生（3日龄）荣昌仔猪而不是成年仔猪（180日龄）中，可以观察到miR-429在皮下脂肪中的表达。这种表达模式表明miR-429可能在功能上与出生后脂肪形成有关。然而，我们目前缺乏对miR-429在前脂肪细胞分化过程中的机制理解。在这项研究中，我们研究了miR-429在猪皮下和肌内前脂肪细胞增殖和分化中的功能。在我们的猪前脂肪细胞分化模型中，miR-429的表达在诱导成脂后显著降低。过表达miR-429可显著下调脂肪生成标记基因PPARγ、aP2、FAS的表达，损害甘油三酯的积累，而脂解基因ATGL的表达不受影响。此外，我们观察到miR-429显著促进了猪前脂肪细胞的增殖。我们还发现miR-429可以直接结合KLF9和p27的3 ' - utr，这两种基因促进前脂肪细胞分化并抑制细胞周期进展。综上所述，我们的数据支持miR-429在调节猪前脂肪细胞分化和增殖中的新作用，而KLF9和p27是miR-429在这些过程中的有效靶点。
MicroRNAs (miRNAs) are crucial regulatory molecules for adipogenesis. They contribute to the controlling of proliferation and differentiation of preadipocytes. Previous studies revealed an important role of miR-429 in cell invasion, migration, and apoptosis. Our previous work has shown that the expression of miR-429 in subcutaneous fat can be observed in newly born (3-day-old) Rongchang piglets rather than their adult counterparts (180-day-old). This expression pattern suggests that miR-429 might be functionally related to postnatal adipogenesis. However, we currently lack a mechanistic understanding of miR-429 within the context of preadipocyte differentiation. In this study, we investigated the function of miR-429 in porcine subcutaneous and intramuscular preadipocyte proliferation and differentiation. In our porcine preadipocyte differentiation model, miR-429 expression decreased remarkably upon adipogenic induction. Overexpression of miR-429 notably down-regulated the expression of adipogenic marker genes: PPARγ, aP2, FAS and impaired the triglyceride accumulation, while the expression of lipolytic gene ATGL was not affected. In addition, we observed that miR-429 significantly promoted the proliferation of porcine preadipocytes. We also found that miR-429 could directly bind to the 3′-UTRs of KLF9 and p27, which have been well documented to promote preadipocyte differentiation and repress cell cycle progression. Taken together, our data support a novel role of miR-429 in regulating porcine preadipocyte differentiation and proliferation, and KLF9 and p27 are potent targets of miR-429 during these processes.
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DOI：
10.12659/msm.893412
发表时间：
2015-02-02
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Lei W;Liu YE;Zheng Y;Qu L
通讯作者：
Qu L
影响因子：
5.6
作者：
Pan, Shifeng;Yang, Xiaojing;Zhao, Ruqian
通讯作者：
Zhao, Ruqian
DOI：
10.1016/j.bbrc.2015.01.145
发表时间：
2015-03-20
影响因子：
3.1
作者：
Escalona-Nandez, Ivonne;Guerrero-Escalera, Dafne;Perez-Monter, Carlos
通讯作者：
Perez-Monter, Carlos
影响因子：
7.1
作者：
Calkins, C. R.;Hodgen, J. M.
通讯作者：
Hodgen, J. M.
影响因子：
12.4
作者：
Pei, H.;Yao, Y.;Wu, J-R
通讯作者：
Wu, J-R