PREPARATION OF DNA-CARRYING AFFINITY LATEX AND PURIFICATION OF TRANSCRIPTION FACTORS WITH THE LATEX
PREPARATION OF DNA-CARRYING AFFINITY LATEX AND PURIFICATION OF TRANSCRIPTION FACTORS WITH THE LATEX
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DOI：
10.1163/156856294x00031
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发表时间：
1994-01-01
影响因子：
3.6
通讯作者：
KAWAGUCHI, H
中科院分区：
文献类型：
--
作者：
INOMATA, Y;WADA, T;KAWAGUCHI, H
我们已经开发了用于分离和纯化转录因子的携带DNA的乳胶颗粒。这些颗粒由苯乙烯（St）、甲基丙烯酸缩水甘油酯（GMA）和二乙烯基苯（DVB）组成。经证实，这些颗粒的乙醇胺处理的表面不发生蛋白质的非特异性吸附。通过共价偶联将序列特异性DNA寡聚物固定到胶乳颗粒。转录因子E4TF3，有效地纯化到同质使用latext颗粒。 相比之下，使用携带DNA的琼脂糖凝胶的纯化产生差的结果。与携带DNA的琼脂糖凝胶相比，乳胶颗粒在从粗核提取物中纯化E4TF 3方面表现出数倍的效率。
We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.