Anorexigenic cocaine- and amphetamine-regulated transcript peptide intensifies fear reactions in rats
Anorexigenic cocaine- and amphetamine-regulated transcript peptide intensifies fear reactions in rats
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DOI：
10.1016/s0006-8993(99)02383-5
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发表时间：
2000-02-28
期刊：
影响因子：
2.9
通讯作者：
Rägo, L
中科院分区：
文献类型：
--
作者：
Kask, A;Schiöth, HB;Rägo, L
越来越多的食欲调节肽被发现。抑制食物摄入的调节剂的列表比食欲刺激剂的列表长得多。在许多情况下，抑制食物摄入的肽促进恐惧反应，而大多数减少焦虑反应的药物刺激食欲。从下丘脑文库中分离出可卡因和安非他明调节转录本 (CART) cDNA，据报道 CART 可以抑制食物摄入并介导瘦素的食欲减退作用。在这里，我们表明，将 CART 的活性核心片段 (CART(89-103)，0.04-5.0 nmol) 注射到侧脑室中不仅会抑制食物摄入，还会引起高架十字迷宫中焦虑样反应的剂量依赖性增加测试。脑室内给予 CART(82-103) (0.04-5.0 nmol) 不会抑制水的摄入，也不会影响旷场测试中的自发运动活动，排除了肽的非特异性作用。我们的结果表明，CART 可能是大脑中调节压力对食欲影响的内源性因素。 (C) 2000 Elsevier Science B.V. 保留所有权利。
An increasing number of appetite-regulating peptides are being discovered. The list of regulators inhibiting food intake is considerably longer than that of appetite stimulators, In many cases, the peptides inhibiting food intake facilitate fear reactions, whereas the majority of the agents reducing anxiety responses stimulate appetite. Cocaine- and amphetamine-regulated transcript (CART) cDNA was isolated from hypothalamic libraries and CART was reported to inhibit food intake and to mediate the anorectic effects of leptin, Here, we show that the active core fragment of CART (CART(89-103), 0.04-5.0 nmol) injected into lateral cerebral ventricle not only inhibits food intake, but also causes a dose-dependent increase in anxiety-like reactions in elevated plus-maze test. Intracerebroventricular administration of CART(82-103) (0.04-5.0 nmol) did not inhibit water intake and did not affect spontaneous locomotor activity in the open field test ruling out unspecific effects of the peptide. Our results suggest that CART could be an endogenous factor in the brain mediating the effects of stress on appetite. (C) 2000 Elsevier Science B.V. All rights reserved.