Hedgehog peptide promotes cell polarization and lumen formation in developing mouse submandibular gland
Hedgehog peptide promotes cell polarization and lumen formation in developing mouse submandibular gland
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DOI：
10.1016/j.bbrc.2005.11.106
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发表时间：
2006-01-20
影响因子：
3.1
通讯作者：
Hieda, Y
中科院分区：
文献类型：
--
作者：
Hashizume, A;Hieda, Y
发育中的小鼠下颌下唾液腺（SMG）的管状形成始于胚胎日(E) 14。Sonic hedgehog基因（Shh）缺失小鼠的SMG最近被证明不能发展到E14以上的阶段。在这里，我们研究了Shh肽对SMG外植体管形成的影响。当培养来自e14小鼠的SMG雏形时，经Shh肽处理的腺的终末芽形成具有管腔的腺泡样结构，而对照腺的终末芽仍为细胞团。在腺的腺泡样顶芽中，紧密连接蛋白ZO-1和claudin-3划定了管腔，顶膜蛋白水通道蛋白5积聚在管腔细胞表面。在处理过的腺体中，层粘连蛋白-5在顶芽基底层的沉积速度加快。提示hedgehog信号通路促进SMG上皮的管腔形成和细胞极化。(c) 2005爱思唯尔公司版权所有。
Tube formation of the developing mouse submandibular salivary gland (SMG) begins at embryonic day (E) 14. The SMG of Sonic hedgehog (Shh) null mice was recently shown to fail to progress to stages beyond around E14. Here, we examined the effects of Shh peptide on tube formation of SMG explants. When the SMG rudiments from E 14 mice were cultured, terminal buds of glands treated with Shh peptide formed the acini-like structure with a lumen whereas those of control glands remained as cell masses. In the acini-like terminal buds of the treated glands, tight junction proteins of ZO-1 and claudin-3 delineated the lumen and the apical membrane protein aquaporin-5 accumulated at the luminal cell surfaces. Moreover, laminin-5 deposition at the basal lamina region of terminal buds was accelerated in treated glands. It is suggested that hedgehog signaling promotes lumen formation and cell polarization of developing SMG epithelium. (c) 2005 Elsevier Inc. All rights reserved.