Automatic analysis of human sperm motion.
Automatic analysis of human sperm motion.
复制标题
自动分析人类精子运动。
DOI：
10.1002/j.1939-4640.1987.tb02428.x
复制
发表时间：
1987
影响因子：
--
通讯作者：
Davis,RO
中科院分区：
文献类型：
--
作者：
Katz,DF;Davis,RO
介绍了一种新的计算机化方法，其中精子运动特征的自动分析。对包含精子图像的视频场进行电子数字化，并确定每个精子头部的质心位置。随着时间的推移，一连串的质心确定了单个精子的游泳路径。详细的路径采集描述了人类精子精液中，并包括一个讨论如何个别细胞被识别和区分彼此。一组多样化的运动特征计算每个精子，包括两个新的措施的基础上的瞬时转向角的路径形状。对传统的和新的活力和游泳模式的措施进行了评估和比较的一致性和冗余。从人类精液的数据分析表明，新的角度措施可能是特别有用的精子表现出广泛不同的运动模式之间的区别。
A new computerized methodology is described in which sperm movement characteristics are analyzed automatically. Video fields containing images of spermatozoa are electronically digitized, and the centroid position of each sperm head is determined. Over time, strings of centroids identify the swimming paths of individual spermatozoa. Details of path acquisition are described for human spermatozoa in semen, and include a discussion of how individual cells are identified and distinguished from each other. A diversified set of movement characteristics is computed for each spermatozoon, including two new measures of path shape based on the instantaneous turning angle. The traditional and the new measures of vigor and swimming pattern are evaluated and compared for consistency and redundancy. Analysis of data from human semen indicates that the new angular measures may be particularly useful in discriminating between spermatozoa exhibiting widely different patterns of motion.