Quantitative chiral analysis of phthaloylglutamic acid and related analogs by a single ratio kinetic method using electrospray ionization and matrix-assisted laser desorption techniques.
Quantitative chiral analysis of phthaloylglutamic acid and related analogs by a single ratio kinetic method using electrospray ionization and matrix-assisted laser desorption techniques.
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DOI：
10.1002/rcm.3405
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
S. Ramagiri;Renuka Gupte;I. Rakov;C. R. Yates;Duane D. Miller
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文献类型：
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作者：
S. Ramagiri;Renuka Gupte;I. Rakov;C. R. Yates;Duane D. Miller
采用电喷雾离子化（ESI）和基质辅助激光解吸技术，建立了Cook动力学法定量分析邻苯二甲酰谷氨酸及其二甲酯的快速方法。通过ESI质谱法生成含有手性邻苯二甲酰谷氨酸及其二甲酯的过渡金属结合络合物离子，并进行碰撞诱导解离。两种竞争性解离速率之比与药物混合物的对映体组成有关。一个七点校准曲线，来自动力学方法，允许快速定量的药物混合物的对映体过量。在本文中，基质辅助激光解吸/电离（MALDI）耦合线性离子阱（LIT）技术的适用性进行了评估，作为一种补充技术，ESI手性歧视和定量。
A rapid method for quantitative chiral analysis of phthaloylglutamic acid and its dimethyl ester by Cook's kinetic method is demonstrated using electrospray ionization (ESI) and matrix-assisted laser desorption techniques. Transition-metal-bound complex ions containing the chiral phthaloylglutamic acid and its dimethyl ester are generated by ESI mass spectrometry and subjected to collision-induced dissociation. The ratio of the two competitive dissociation rates is related to the enantiomeric composition of the drug mixture. A seven-point calibration curve, derived from the kinetic method, allowed rapid quantitation of the enantiomeric excess of drug mixtures. In this paper, matrix-assisted laser desorption/ionization (MALDI) coupled with the linear ion trap (LIT) technique is evaluated for its applicability as a complementary technique to ESI for chiral discrimination and quantitation.