Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome
Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome
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DOI：
10.1002/art.1780390816
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发表时间：
1996-08-01
影响因子：
--
通讯作者：
Nomoto, K
中科院分区：
文献类型：
--
作者：
Ohyama, Y;Nakamura, S;Nomoto, K
目的：通过检测自身免疫损伤部位细胞因子的产生模式，研究干燥综合征（SS）的局部自身免疫损伤的开始和进展。干燥综合征是一种自身免疫疾病，也被认为是一种淋巴细胞侵袭性疾病。采用聚合酶链反应方法对15例SS患者唇唾液腺细胞因子信使RNA （mRNA）表达进行了检测，并对唇唾液腺浸润淋巴细胞进行了免疫组化检测。结果：所有患者均能检测到Th1细胞因子的信使RNA，如白细胞介素-2 （IL-2）和干扰素- γ，而部分患者仅检测到Th2细胞因子mRNA，如IL-4和IL-5；在所有患者中也一致检测到由多种细胞类型产生的其他细胞因子mRNA，包括IL-10、IL-6和转化生长因子β (TGF β)，而在一些患者中检测到IL-12 mRNA tases。结论：这些结果表明Th1细胞因子以及IL-10、IL-6和TGF β在诱导和/或维持SS中至关重要。而Th2细胞因子参与疾病的发展过程，特别是局部B细胞活化。
Objective, To characterize the initiation and progress of localized autoimmune damage in Sjogren's syndrome (SS), an autoimmune disease that is also considered to be a lymphoaggressive disorder, by examining the pattern of cytokine production at the site of autoimmune damage,Methods. Using a polymerase chain reaction-based method, cytokine messenger RNA (mRNA) expression in the labial salivary glands of 15 patients with SS was investigated In addition, the infiltrating lymphocytes in the labial salivary glands were examined immunohistochemically,Results, Messenger RNAs of Th1 cytokines, such as interleukin-2 (IL-2) and interferon-gamma, were consistently detected in all patients, while Th2 cytokine mRNAs, such as IL-4 and IL-5, mere detected in some cases, in association with strong B cell accumulation in the labial salivary glands, Other cytokine mRNAs produced by a variety of cell types, including IL-10, IL-6, and transforming growth factor beta (TGF beta), were also consistently detected in all patients, while IL-12 mRNA tvas detected in some of the patients,Conclusion, These results suggest that Th1 cytokines, as well as IL-10, IL-6, and TGF beta, are essential in the induction and/or maintenance of SS, while Th2 cytokines are involved in the progression of the disease process, especially local B cell activation.