A novel DNA-binding motif in MarA: The first structure for an AraC family transcriptional activator
A novel DNA-binding motif in MarA: The first structure for an AraC family transcriptional activator
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DOI：
10.1073/pnas.95.18.10413
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发表时间：
1998-09-01
影响因子：
11.1
通讯作者：
Davies, DR
中科院分区：
文献类型：
--
作者：
Rhee, S;Martin, RG;Davies, DR
描述了AraC原核转录激活因子家族成员MarA与其同源DNA结合位点复合的晶体结构。MarA由两个相似的亚结构域组成，每个亚结构域含有螺旋-转角-螺旋DNA结合基序。基序的两个识别螺旋被插入DNA的同一面上的相邻大沟区段中，但仅相隔27埃，从而使DNA弯曲约35度。识别螺旋与DNA大沟之间广泛的相互作用提供了序列特异性。
A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 Angstrom thereby bending the DNA by approximate to 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.