Discovering the mechanisms of strain-dependent response of Staphylococcus aureus to photoinactivation: Oxidative stress toleration, endogenous porphyrin level and strain's virulence
Discovering the mechanisms of strain-dependent response of Staphylococcus aureus to photoinactivation: Oxidative stress toleration, endogenous porphyrin level and strain's virulence
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DOI：
10.1016/j.pdpdt.2013.02.004
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发表时间：
2013-12-01
影响因子：
3.3
通讯作者：
Grinholc, Mariusz
中科院分区：
文献类型：
--
作者：
Kossakowska, Monika;Nakonieczna, Joanna;Grinholc, Mariusz
背景：金黄色葡萄球菌通常被认为对光灭活敏感。然而，它的应变依赖性的光动力学处理的现象已被报道。此外，根据photoinactivation.Methods：这项工作的目的是调查细菌的耐受性的氧化应激，卟啉水平，光敏剂的摄取和菌株的毒力的研究甲氧西林敏感和耐甲氧西林S。金葡菌菌株及其对光动力灭活（使用原卟啉二精氨酸盐、甲苯胺蓝0和5-氨基乙酰丙酸）的响应。然而，我们已经证明，由PPArg（2）、ALA和TBO组成的多组分敏化剂可以消除S。金黄色葡萄球菌提高了对光灭活的抗性，并且高毒力和低毒力S.金黄色葡萄球菌菌株可以很容易地用PDI根除。此外，我们已经表明，光动力灭活可以降低毒力的S。结论：菌株依赖性光灭活反应的机制是复杂的，多因素的，但使用几种敏化剂的光动力治疗的阻力升高可以忽略。(C)2013爱思唯尔有限公司版权所有。
Background: Staphylococcus aureus is generally known to be susceptible to photoinactivation. However, the phenomenon of its strain-dependent response to photodynamic treatment has been reported. Moreover, the factors determining the emerging variation among strains according to photoinactivation remain unclear.Methods: This work aimed to investigate any relevant correlation between bacterial toleration of oxidative stress, porphyrin level, photosensitizer uptake and strain's virulence of studied methicillin-susceptible and methicillin-resistant S. aureus strains and their response to photodynamic inactivation (using protoporphyrin diarginate, toluidine blue 0 and 5-aminolevulinic acid).Results: Obtained data let to demonstrate that studied factors have limited impact on strain response to PDI. However, we have shown that multicomponent sensitizing agent i.e. consisted of PPArg(2), ALA and TBO would eliminate the S. aureus elevated resistance to photoinactivation and that both highly virulent and low virulent S. aureus strains could be easily eradicated with the use of PDI. Moreover, we have shown that photodynamic inactivation could decrease the virulence of S. aureus extracellular fraction.Conclusion: The mechanism underlying strain-dependent response to photoinactivation is complex and multifactorial nevertheless with the use of several sensitizing agents the elevated resistance to photodynamic treatment can be omitted. (C) 2013 Elsevier B.V. All rights reserved.