Experimental study on TGF-1-mediated CD147 expression in oral submucous fibrosis
Experimental study on TGF-1-mediated CD147 expression in oral submucous fibrosis
复制标题
TGF-1介导的CD147表达在口腔粘膜下纤维化中的实验研究
DOI：
10.1111/odi.12845
复制
发表时间：
2018-09-01
期刊：
影响因子：
3.8
通讯作者：
Su, T.
中科院分区：
文献类型：
--
作者：
Wang, W.;Xiong, H.;Su, T.
尽管先前的证据表明CD 147与器官纤维化的进展密切相关，并且各种信号通路已被证明调节其表达，但CD 147在口腔粘膜下纤维化（OSF）中的作用仍在很大程度上未知。我们通过免疫组织病理学研究了CD 147和转化生长因子1（TGF-1）在OSF组织阵列的人类样本中的表达。采用Pearson相关分析探讨CD 147与TGF-1的相关性。应用免疫荧光和Western blotting技术检测人口腔角质形成细胞（HOKs）中CD 147的表达水平，并分别用TGF-1或TGF-1受体抑制剂LY 2157299和槟榔碱刺激HOKs中CD 147的表达。此外，CD 147水平与纤维化分期显着相关。槟榔碱处理后，CD 147表达上调主要与TGF-1信号通路有关，而抑制TGF-1则下调CD 147的表达。结论槟榔碱通过TGF-1信号通路促进HOKs中CD 147的表达，而CD 147的过度表达可能促进OSF的进展。
T ObjectiveAlthough previous evidence indicates that CD147 is closely involved in the progression of organ fibrosis and various signaling pathways have been proven to regulate its expression, the role of CD147 in oral submucous fibrosis (OSF) remains largely unknown.MethodsIn this study, we investigated the expression of CD147 and transforming growth factor 1 (TGF-1) in human samples of an OSF tissue array by immunohistopathology. Pearson's correlation analysis was conducted to explore the correlation between CD147 and TGF-1. Immunofluorescence and Western blotting were used to investigate to levels of CD147 in Human Oral Keratinocytes (HOKs) followed by TGF-1 or LY2157299, an inhibitor of TGF-1 receptor and arecoline stimulation.ResultsWe found that CD147 was highly expressed in both HOKs and the fibrotic oral mucosa and that this expression was correlated with TGF-1 expression. Additionally, CD147 levels were significantly associated with the fibrosis stage. The TGF-1 signaling pathway was found to be mainly responsible for CD147 up-regulation after arecoline treatment whereas inhibition of TGF-1 down-regulated CD147 expression.ConclusionOur findings suggest arecoline promotes CD147 expression via the TGF-1 signaling pathway in HOKs, whereas overexpression of CD147 may promote OSF progression.