A tomato glutaredoxin gene SlGRX1 regulates plant responses to oxidative, drought and salt stresses
A tomato glutaredoxin gene SlGRX1 regulates plant responses to oxidative, drought and salt stresses
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DOI：
10.1007/s00425-010-1271-1
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发表时间：
2010-11-01
期刊：
影响因子：
4.3
通讯作者：
Zhou, Xueping
中科院分区：
文献类型：
--
作者：
Guo, Yushuang;Huang, Changjun;Zhou, Xueping
Glutaredoxins （Grxs）是一种普遍存在的小热稳定二硫氧化还原酶，在植物发育和氧化胁迫反应中起着至关重要的作用。本文从番茄中分离到一个新的cDNA片段（SlGRX1），该片段编码一个含有共识Grx家族结构域和CGFS活性位点的蛋白，并对其进行了表征。Southern blot分析表明，SlGRX1基因在番茄基因组中为单拷贝。实时荧光定量RT-PCR分析显示，SlGRX1在番茄叶、根、茎和花中普遍表达，其表达可受氧化、干旱和盐胁迫诱导。病毒诱导的SlGRX1基因沉默介导的番茄SlGRX1基因沉默导致番茄对氧化和盐胁迫的敏感性增加，叶绿素相对含量降低，对干旱胁迫的耐受性降低，相对含水量降低。相比之下，SlGRX1在拟南芥中的过表达显著提高了植物对氧化、干旱和盐胁迫的抗性。此外，通过实时荧光定量PCR分析，在slgrx1过表达的拟南芥植株中，氧化、干旱和盐胁迫相关基因Apx2、Apx6和RD22的表达水平上调。我们的研究结果表明，Grx基因SlGRX1在调节抗氧化、干旱和盐胁迫的非生物耐受性中起重要作用。
Glutaredoxins (Grxs) are ubiquitous small heat-stable disulfide oxidoreductases that play a crucial role in plant development and response to oxidative stress. Here, a novel cDNA fragment (SlGRX1) from tomato encoding a protein containing the consensus Grx family domain with a CGFS active site was isolated and characterized. Southern blot analysis indicated that SlGRX1 gene had a single copy in tomato genome. Quantitative real-time RT-PCR analysis revealed that SlGRX1 was expressed ubiquitously in tomato including leaf, root, stem and flower, and its expression could be induced by oxidative, drought, and salt stresses. Virus-induced gene silencing mediated silencing of SlGRX1 in tomato led to increased sensitivity to oxidative and salt stresses with decreased relative chlorophyll content, and reduced tolerance to drought stress with decreased relative water content. In contrast, over-expression of SlGRX1 in Arabidopsis plants significantly increased resistance of plants to oxidative, drought, and salt stresses. Furthermore, expression levels of oxidative, drought and salt stress related genes Apx2, Apx6, and RD22 were up-regulated in SlGRX1-overexpressed Arabidopsis plants when analyzed by quantitative real-time PCR. Our results suggest that the Grx gene SlGRX1 plays an important role in regulating abiotic tolerance against oxidative, drought, and salt stresses.