HUMIG - A NEW HUMAN MEMBER OF THE CHEMOKINE FAMILY OF CYTOKINES
HUMIG - A NEW HUMAN MEMBER OF THE CHEMOKINE FAMILY OF CYTOKINES
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DOI：
10.1006/bbrc.1993.1403
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发表时间：
1993-04-15
影响因子：
3.1
通讯作者：
FARBER, JM
中科院分区：
文献类型：
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作者：
FARBER, JM
小鼠巨噬细胞中淋巴因子激活基因的表征导致先前鉴定Mumig，Mumig是一种干扰素-γ-诱导的鼠基因，其编码细胞因子趋化因子家族的成员。MumigcDNA探针用于筛选从已用干扰素-γ处理的THP-1人单核细胞系的培养物制备的cDNA文库。这导致了Humig的鉴定，Humig是趋化因子基因家族的一个新的人类成员。在THP-1细胞和外周血单个核细胞中，干扰素-γ可诱导腐殖酸，但干扰素-α或脂多糖不诱导腐殖酸。对小鼠和人类基因组DNA的分析表明，Mumigand Humiggenes是真正的小鼠-人类同源物。HumigmRNA编码预测的103个残基的分泌型HuMig蛋白，Mr 11，725。
Characterization of lymphokine-activated genes in mouse macrophages led to the identification previously of Mumig, an interferon-γ-inducible murine gene that encodes a member of the chemokine family of cytokines. The MumigcDNA probe was used to screen a cDNA library prepared from cultures of the THP-1 human monocytic cell line that had been treated with interferon-γ. This led to the identification of Humig, a new human member of the chemokine gene family. Humigis induced in THP-1 cells and in peripheral blood mononuclear cells by interferon-γ but not by interferon-α or by lipopolysaccharide. Analysis of mouse and human genomic DNAs suggested that the Mumigand Humiggenes are true mouse-human homologues. The HumigmRNA encodes a predicted secreted HuMig protein of 103 residues, Mr11,725.