Variation of Hematocrit Values of Rainbow Trout Blood Samples Incubated under Different Conditions
Variation of Hematocrit Values of Rainbow Trout Blood Samples Incubated under Different Conditions
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不同条件下虹鳟鱼血样血细胞比容值的变化
DOI：
10.2331/suisan.43.301
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
H. Kawatsu
中科院分区：
文献类型：
--
作者：
T. Nomura;H. Kawatsu
研究了在不同温度、血样体积和肝素钠浓度条件下孵育的虹鳟鱼血样的血细胞比容值的变化。根据实验目的，用肝素处理的注射器从尾动脉抽取血样，并转移至两个或三个试管（7×70mm）中。每个样本在零时和30、60、90、120和180分钟后测定血细胞比容值。在每种条件下测量十个样品。实验1和实验2研究了温度的影响，温度越低，血细胞比容值的变化越小，在0.3°C下，出血后读数保持稳定60分钟。在实验3中，研究了试管中血液体积的影响。每份血量分为三份，体积分别为0.3、0.6和0.9ml。随着试管中血容量的增加，血细胞比容值趋于变高。在实验4中，研究了肝素浓度的影响。在每毫升血液0.2-0.5mg肝素钠范围内，未发现肝素浓度对血细胞比容值的影响。
Variation of hematocrit values was studied on rainbow trout blood samples incubated under different conditions of temperature, volume of blood sample and concentration of heparin sodium. Blood samples were taken from the caudal artery with heparintreated syringes and transferred to two or three test tubes (7×70mm) in accordance with the purpose of the experiment.. Hematocrit values were determined at zero-time and after 30, 60, 90, 120 and 180 minutes for each sample. Ten samples were measured under each condition. In Experiments 1 and 2, the effect of temperature was studied, change in hematocrit values became smaller at lower temperatures, and at 0.3°C the reading remained stable for 60 minutes after bleeding. In experiment 3, the effect of the volume of blood in the test tube was studied. Each blood ample was divided into three parts, with volumes of 0.3, 0.6 and 0.9ml. Hematocrit values tended to become higher with the increase in blood volume in the test tube. In Experiment 4, the effect of heparin concentration was studied. Within the range of 0.2-0.5mg heparin sodium per ml of blood, no effects of heparin concentration on hematocrit value were found.