Detection and characterization of a plastid envelope DNA‐binding protein which may anchor plastid nucleoids.
Detection and characterization of a plastid envelope DNA‐binding protein which may anchor plastid nucleoids.
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DOI：
10.1002/j.1460-2075.1993.tb05687.x
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发表时间：
1993-02
期刊：
影响因子：
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通讯作者：
Naoki Sato;C. Albrieux;J. Joyard;Roland Douce;Tsuneyoshi Kuroiwa
中科院分区：
文献类型：
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作者：
Naoki Sato;C. Albrieux;J. Joyard;Roland Douce;Tsuneyoshi Kuroiwa
在豌豆幼叶中，叶绿体DNA(CpDNA)结合在叶绿体(叶绿体)主动分裂的被膜上。用西南印迹法鉴定和鉴定参与cpDNA与包膜结合的蛋白质。叶绿体(叶绿体)内包膜中的130 kDa蛋白质与cpDNA内的特定序列结合。这些序列包括位于psaAB基因上游的0.41kbP序列、位于PetA基因下游的0.57kbP序列和位于rpoC2基因内的1.2kbP序列。荧光显微镜观察到cpDNA位于叶绿体外围的豌豆幼叶被膜中检测到该蛋白，而在成熟叶片中检测不到该蛋白。因此，我们推测130 kDa蛋白参与了cpDNA与被膜的结合，并将其命名为叶绿体被膜DNA结合蛋白。
Chloroplast DNA (cpDNA) binds to the envelope membrane of actively dividing chloroplasts (plastids) in young pea leaves. South‐western blotting was used to identify and characterize the protein involved in the binding of cpDNA to the envelope membrane. A 130 kDa protein in the inner chloroplast (plastid) envelope membrane binds specific sequences within the cpDNA. These included a 0.41 kbp sequence located upstream of the psaAB gene, a 0.57 kbp sequence located downstream of the petA gene and a 1.2 kbp sequence located within the rpoC2 gene. The protein was detected in the envelope membrane of young pea leaves in which the cpDNA had been located by fluorescence microscopy at the chloroplast periphery, whereas it was undetectable in mature leaves. We therefore propose that the 130 kDa protein is involved in the binding of cpDNA to the envelope membrane, and named it plastid envelope DNA‐binding protein.