Sulfur-containing proteins and epidermal keratinization.
Sulfur-containing proteins and epidermal keratinization.
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DOI：
10.1083/jcb.40.3.830
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发表时间：
1969-03
期刊：
影响因子：
--
通讯作者：
Epstein WL
中科院分区：
文献类型：
--
作者：
Fukuyama K;Epstein WL
在注射硫35标记的胱氨酸后，用放射自显影法研究了角化上皮中含硫蛋白的合成位点。Bern等人(3)证明了银颗粒在角化层下方的浓度，这表明在头发角化的最后阶段添加了大量的硫。相比之下，B6langer(4)报道蛋白质合成主要发生在Malpighian细胞中，Hoober和Bernstein（17）也得出了类似的结论。用硫35的β射线发射制备的射线照相底片缺乏分辨率，用硫35精确定位放射性变得非常困难，因为它的能量（167 Kev）比氚（18 Kev）更大（24）。此外，光显微镜还不足以研究新合成蛋白质的分布和运动，
Sites of synthesis of sulfur-containing protein in keratinizing epithelium have been studied by radioautography after injection of sulfur35-labeled cystine. Bern et al.(3) demonstrated concentration of silver grains immediately below the cornified layer, suggesting that much of sulfur is added at the final stages of keratinization as observed in hair. In contrast, B6langer (4) reported that protein synthesis occurs mainly in the Malpighian cells, and Hoober and Bernstein (17) reached a similar conclusion. Radioautographs prepared with beta emission of sulfur35 lack resolution, and determination of precise localization of radioactivity with sulfur3 5 becomes very difficult because of its greater energy (167 Kev) compared to that of tritium (18 Kev)(24). In addition, light microscopy alone is not sufficient to study distribution and movement of newly synthesized protein,