Molecular markers associated with plant architecture and resistance to common blight, web blight, and rust in common beans
Molecular markers associated with plant architecture and resistance to common blight, web blight, and rust in common beans
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DOI：
10.21273/jashs.121.5.794
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发表时间：
1996-09-01
影响因子：
1.9
通讯作者：
Kaeppler, SM
中科院分区：
文献类型：
--
作者：
Jung, GW;Coyne, DP;Kaeppler, SM
利用随机扩增多态性DNA（RAPD）标记构建了菜豆（Phaseolus vulgaris L.）BAC 6 × HT 7719杂交研究菜豆抗病性遗传。该连锁图跨度为545 cM，包括本研究中使用的84个标记中的75个。对128个重组自交系的群体进行了对普通白叶枯病的抗性、对网枯病的叶片抗性[WB; Thanatephorus cucumeris（Frank）Donk]和对锈病的抗性[Uromyces approxulatus var，approxulatus（E. coli：Pers）Unger]。白叶枯病[CBB;野油菜黄单胞菌致病变种（Xanthomonascampestris pv.）评价了CBB株Epif-IV在后期发育的三叶叶中和CBB株EK-11在种子、第一片三叶叶、后期发育的三叶叶和豆荚中的菜豆[Phaseoli（Smith）Dye]抗性。另外，对株高和分支密度进行了评价，每个性状的表型变异由2 ~ 6个标记解释14%~ 34%，其中14个标记位点和9个未定位标记中的7个标记位点与性状间存在显著的关联，检测到的QTL分布呈现非随机性，多数显著标记与多个性状相关或与标记紧密连锁一个标记BC 409（1250）与WE抗性、在发育较晚的三叶叶中对CBB菌株Epif-IV的抗性以及在第一片三叶叶、发育较晚的三叶叶和豆荚中对CBB菌株EK-11的抗性显著相关。在RAPD标记H19（1050）和标记AJ16（250）的14.6chi和12.5cM之间定位了一个抗锈基因。
Random amplified polymorphic DNA (RAPD) markers were used to construct a partial linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross BAC 6 x HT 7719 for studying the genetics of disease resistance in common bean. The linkage map spanned 545 cM and included 75 of 84 markers used in this study, The population of 128 recombinant inbred lines was evaluated for resistance to common bacterial blight, foliar resistance to web blight [WB; Thanatephorus cucumeris (Frank) Donk], and resistance to rust [Uromyces appendiculatus var, appendiculatus (Pers.:Pers) Unger]. Common bacterial blight [CBB; Xanthomonas campestris pv. phaseoli (Smith) Dye] resistance was evaluated for CBB strain Epif-IV in later-developed trifoliolate leaves and for CBB strain EK-11 in seeds, first trifoliolate leaves, later-developed trifoliolate leaves, and pods. In addition,lines were rated for plant uprightness and branch density, Two to six markers accounted for 14% to 34% of the phenotypic variation for each trait, Significant marker locus-trait associations were found for 14 mapped loci and 7 of the 9 unmapped markers, The distribution of detected QTL appeared to be nonrandom with most significant markers associated with more than one trait of closely linked to markers significantly associated with variation for a different trait, One marker, BC409(1250), was significantly associated with WE resistance, resistance for CBB strain Epif-IV in later-developed trifoliolate leaves, and resistance for CBB strain EK-11 in first trifoliolate leaves, later-developed trifoliolate leaves, and pods. A rust resistance gene was mapped in an interval 14.6 chi from RAPD marker H19(1050) and 12.5 cM from marker AJ16(250).