Association of lipoprotein lipase Hind III and Ser 447 Ter polymorphisms with dyslipidemia in Asian Indians.
Association of lipoprotein lipase Hind III and Ser 447 Ter polymorphisms with dyslipidemia in Asian Indians.
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DOI：
10.1016/j.amjcard.2005.11.056
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发表时间：
2006-05
期刊：
影响因子：
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通讯作者：
V. Radha;V. Mohan;Ramprakash Vidya;A. K. Ashok;R. Deepa;R. Mathias
中科院分区：
文献类型：
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作者：
V. Radha;V. Mohan;Ramprakash Vidya;A. K. Ashok;R. Deepa;R. Mathias
研究表明，在某些人群中，脂蛋白脂酶基因与血脂异常和动脉粥样硬化之间存在关联。本研究的目的是探讨常见脂蛋白脂酶HindIII(T-G)和Ser447Ter(C-G)基因多态性与亚洲印度人血脂异常的关系。共有1,015名受试者，包括550名正常糖耐量受试者和465名2型糖尿病患者，从金奈城乡流行病学研究中随机挑选出来。用酶法检测血清总胆固醇、高密度脂蛋白胆固醇和血清甘油三酯水平。使用Friedewald公式计算低密度脂蛋白胆固醇。采用聚合酶链式反应-限制性片段长度多态性方法进行基因分型。研究发现，HindIII的H+等位基因与低高密度脂蛋白胆固醇和高甘油三酯水平之间存在显著关联。Ser447Ter的Ser等位基因也与低高密度脂蛋白水平密切相关。未发现H+等位基因和Ser等位基因与总或低密度脂蛋白胆固醇水平相关。使用期望最大化算法生成组内单倍型频率，以检测病例对照组之间总体单倍型频率分布的差异。单倍型分析显示，H+丝氨酸和H HDLTer分别是低−胆固醇和高甘油三酯水平的高危和低危单倍型。结论：在亚洲印度人中，脂蛋白脂酶基因的H+Ser单倍型与低高密度脂蛋白胆固醇水平和高甘油三酯血症相关。
Studies have shown an association between the lipoprotein lipase gene and dyslipidemia and atherosclerosis in some populations. The aim of this study was to investigate the association between the common lipoprotein lipase HindIII (T-G) and Ser447Ter (C-G) polymorphisms with dyslipidemia in Asian Indians, who are known to have very high rates of premature coronary artery disease. A total of 1,015 subjects, comprising 550 normal glucose-tolerant subjects and 465 patients with type 2 diabetes, were randomly selected from the Chennai Urban Rural Epidemiology Study. The total serum cholesterol, high-density lipoprotein (HDL) cholesterol, and serum triglyceride levels were assayed using enzymatic methods. Low-density lipoprotein cholesterol was calculated using the Friedewald formula. Genotyping was done using the polymerase chain reaction-restriction fragment length polymorphism method. A significant association was found between the H+ allele of HindIII with low HDL cholesterol and elevated triglyceride levels. The Ser allele of Ser447Ter was also strongly associated with low HDL cholesterol levels. No association was found between the H+ allele and Ser Allele with the total or low-density lipoprotein cholesterol levels. Group-wise haplotype frequencies were generated using the expectation-maximization algorithm to detect differences in overall haplotype frequency profiles between the case-control groups. The haplotype analysis showed that the H+ Ser and H− Ter were the “high-risk” and “low-risk” haplotypes for low HDL cholesterol and elevated triglyceride levels, respectively. In conclusion, the H+ Ser haplotype of the lipoprotein lipase gene was associated with low HDL cholesterol levels and hypertriglyceridemia in Asian Indians.