INHIBITION OF ECO-RI ACTION BY POLYNUCLEOTIDES - A CHARACTERIZATION OF THE NONSPECIFIC-BINDING OF THE ENZYME TO DNA
INHIBITION OF ECO-RI ACTION BY POLYNUCLEOTIDES - A CHARACTERIZATION OF THE NONSPECIFIC-BINDING OF THE ENZYME TO DNA
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DOI：
10.1093/nar/8.20.4727
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
MAASS, G
中科院分区：
文献类型：
--
作者：
LANGOWSKI, J;PINGOUD, A;MAASS, G
The cleavage of the plasmid pBR322 by the restriction endonuclease Eco RI has been studied in the presence of various polynucleotides and the double stranded octanucleotide d-(GGAATTCC) in order to clarify whether there is a preferential interaction of Eco RI with DNA sequences other than -GAATTC-. The steady state kinetic analysis shows that all polynucleotides investigated with the possible exception of poly-dG·poly-dC inhibit the cleavage competitively with Kjvalues in the range of 10−410−5[M nucleotides] The Kjof d-(GGAATTCC is 1.5·10−6[M nucleotides], indicating that the specific binding is approx. 2 orders of magnitude stronger than non-specific binding.
