Characterization and selectivity studies of molecular imprinted membranes of Puerarin using scanning electron microscopy.
Characterization and selectivity studies of molecular imprinted membranes of Puerarin using scanning electron microscopy.
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DOI：
10.1002/sca.20212
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Peiyong Qin;Jie Yang;M. N. Karim
中科院分区：
文献类型：
--
作者：
Peiyong Qin;Jie Yang;M. N. Karim
Molecular-imprinted membranes of Puerarin were prepared by phase inversion technique with acrylonitrile-acrylic acid copolymer (P (AN-co-AA)). To characterize P (AN-co-AA), ubbelohde viscometer was used to measure its viscosity-molecular weight. P (AN-co-AA) with different molecular weight was used to prepare membranes. The copolymer-dimethyl sulfoxide solution with Puerarin (PU) template was coagulated in water at various temperatures. The increase in P (AN-co-AA) molecular weight and the decrease in coagulation temperature caused an increase in PU recognition property of the resultant membrane. The PU imprinted membrane prepared with P (AN-co-AA) showed good selective ability to PU. The purity of PU increased from 56.51 to 98.41 wt%. Surface and cross-section morphology of the membranes were analyzed by using scanning electron micrograph. High-performance liquid chromatography was used for the quantification of Puerarin in isolated fraction.
