A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes
A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes
复制标题
DOI：
10.1007/s11248-014-9800-5
复制
发表时间：
2014-08-01
影响因子：
3
通讯作者：
Abe, Fumitaka
中科院分区：
文献类型：
--
作者：
Ashikawa, Ikuo;Mori, Masahiko;Abe, Fumitaka
种子休眠是一个重要的农艺性状：低水平的休眠会导致过早萌发，而过高的休眠水平会抑制均匀的萌发。作为一种控制作物种子休眠程度的方法，我们使用了与小麦Dog1类似的基因作为转基因。Dog1是拟南芥的一个基因，它是种子休眠的自然变异的基础。我们之前的研究表明，虽然它们与DOG1的序列相似性很低，但一些谷类DOG1基因增强了拟南芥的种子休眠。在此，我们将小麦的TaDOG1L4和大麦的HvDOG1L1两个类似DOG1的基因分别导入小麦品种菲尔德。在玉米泛素启动子的控制下，它们的过度表达提高了种子的休眠水平，而其他性状保持不变。TaDOG1L4比HvDOG1L1更有效，这与先前揭示的这两个基因在拟南芥种子休眠中的作用差异是一致的。利用双链RNA干扰技术将内源TaDOG1L4基因导入田间，可使种子休眠程度降低几十个百分点。这一结果表明，小麦固有的某种程度的种子休眠是由类似Dog1的基因遗传的。
Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in seed dormancy. We previously showed that although their sequence similarities to DOG1 were low, some cereal DOG1-like genes enhanced seed dormancy in Arabidopsis. Here, we introduced two DOG1-like genes, TaDOG1L4 from wheat and HvDOG1L1 from barley, individually into the wheat cultivar Fielder. Their overexpression under the control of a maize ubiquitin promoter enhanced the seed dormancy level while leaving other traits unchanged. TaDOG1L4 was more effective than HvDOG1L1, which accords with the previously revealed difference in the effectiveness of these two genes in Arabidopsis seed dormancy. Knockdown of endogenous TaDOG1L4 in Fielder using double-strand RNA interference decreased the seed dormancy level by several tens of percent. This result indicates that some degree of seed dormancy inherent in wheat is imparted by DOG1-like genes.