Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean)
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean)
复制标题
DOI：
10.4319/lom.2012.10.483
复制
发表时间：
2012-07
期刊：
影响因子：
--
通讯作者：
X. Xing;H. Claustre;S. Blain;F. D’Ortenzio;D. Antoine;J. Ras;C. Guinet
中科院分区：
文献类型：
--
作者：
X. Xing;H. Claustre;S. Blain;F. D’Ortenzio;D. Antoine;J. Ras;C. Guinet
作为叶绿素a （Chl a）浓度的代表，在南大洋克尔盖伦地区用仪器象海豹测量了数千个荧光谱图。为了准确地检索体内荧光仪获得的Chl a浓度，采用两步程序：1)利用高效液相色谱（HPLC）分析进行部署前的相互校准，准确测定Chl - A浓度，不仅可以校准适当浓度单位的荧光，还可以大大减少荧光计之间的差异；2)逐谱猝灭校正分析，有效消除了中午前后表面的荧光猝灭问题，并在白夜之间获得一致的谱图。猝灭校正是通过深荧光值向表面的外推进行的。西地中海的验证程序证明，在没有可靠参考资料的情况下，特别是在像南大洋这样的深层混合水域，这种校正方法是实用和相对可靠的。即使在较浅的混合水中，该方法也能有效降低淬火的影响。
As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two‐step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile‐by‐profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching.