Molecular cytogenetic identification of newly synthetic Triticum kiharae with high resistance to stripe rust
Molecular cytogenetic identification of newly synthetic Triticum kiharae with high resistance to stripe rust
复制标题
新合成高抗条锈病小麦的分子细胞遗传学鉴定
DOI：
10.1007/s10722-018-0649-y
复制
发表时间：
2018-08-01
影响因子：
2
通讯作者：
Zhang, Lianquan
中科院分区：
文献类型：
--
作者：
Liu, Xiaojuan;Zhang, Minghu;Zhang, Lianquan
六个新的双倍体，毛原小麦。和米古施。(2n= 6x = 42，A（t）A（t）GGDD）。它们是由提莫菲维小麦（Triticum timopheevii Zhuk.）（A（t）A（t）GG）和粗山羊草（DD）。这些双倍体表现出68-80%的高育性水平，并在减数分裂中期I表现出相对正常的染色体配对。T.使用寡核苷酸探针Oligo-pSc 119.2-1、Oligo-pTa 535 -1和Oligo-pTa 71 -2的组合，通过荧光原位杂交可以鉴定kiharae。T.在苗期和成株期，Kiharae对条锈病优势小种CYR 34、CYR 31、CYR 32、CYR 33和SY 11 -4表现高抗。然而，来自Ae. tauschii亲本在T. kiharae背景。这些T。kiharae系为拓宽普通小麦基因库的遗传多样性提供了新的材料。
Six new amphiploids, Triticum kiharae Dorof. et Migusch. (2n = 6x = 42, A(t)A(t)GGDD), are described in this study. They were developed by the chromosome doubling of F-1 hybrid crosses between Triticum timopheevii Zhuk. (A(t)A(t)GG) with high resistance to stripe rust and Aegilops tauschii Cosson (DD) by colchicine treatment. These amphiploids showed a high level of fertility of 68-80% and exhibited relatively normal chromosome pairing in meiotic metaphase I. Individual chromosomes of T. kiharae could be identified by multicolor fluorescence in situ hybridization using the combination of oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-pTa71-2. T. kiharae exhibited high resistance to predominant stripe rust races CYR34, CYR31, CYR32, CYR33, and SY11-4 both during the seedling and adult stages. However, high molecular weight glutenin subunits from Ae. tauschii parents were only partially expressed in the T. kiharae background. These T. kiharae lines provide novel materials to widen the genetic diversity of the common wheat gene pool.