Early nephron formation in the developing mouse kidney
Early nephron formation in the developing mouse kidney
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DOI：
10.1046/j.1469-7580.2001.19940385.x
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发表时间：
2001-10-01
影响因子：
2.4
通讯作者：
Sellers, WI
中科院分区：
文献类型：
--
作者：
Bard, JBL;Gordon, A;Sellers, WI
本文用三维共聚焦显微镜观察了小鼠肾脏导管尖端周围凝聚后肾间质的NCAM-和pax2阳性帽（4-5个细胞深）是如何形成肾源性聚集体的。6-8个细胞的聚集体首先出现在E12.5-12.75，紧挨着这个帽，靠近管道表面。随着尖端的推进，NCAM的表达维持在帽中，但仅限于细胞迅速上皮化的聚集体，形成侵入导管上皮的小管。Pax2表达研究显示肾源性胚细胞的表皮是如何形成的：当导管尖端向肾表面延伸时，相关的Pax2+细胞在肾表面形成细胞斑块。这些观察结果修正了我们对肾元起始时间和过程的认识。
This paper reports 3-dimensional confocal microscopy observations on how nephrogenic aggregates form from the NCAM- and Pax2-positive caps (4-5 cells deep) of condensed metanephric mesenchyme surrounding the duct tips of the mouse kidney. Aggregates of 6-8 cells are first seen at similar to E12.5-12.75 immediately proximal to this cap, closely abutting the duct surface. As the tip advances, NCAM expression is maintained in the cap but is otherwise restricted to aggregates whose cells rapidly epithelialise, forming tubules that invade the duct epithelium. Pax2 expression studies shows how the rind of nephrogenic blastemal cells forms: as duct tips extend towards the kidney surface, the associated Pax2+ cells form patches of cells on the kidney surface. These observations revise our knowledge of the timing and process of nephron initiation.