Iron regulatory elements (IREs): a family of mRNA non-coding sequences.
Iron regulatory elements (IREs): a family of mRNA non-coding sequences.
复制标题
铁调节元件 (IRE)：mRNA 非编码序列家族。
DOI：
10.1042/bj3040001
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Theil,EC
中科院分区：
文献类型：
--
作者：
Theil,EC
DNA 中相关顺式调节元件家族创造了一个反复出现的主题，可以通过糖皮质激素受体元件（例如 Diamond 等，1990；O'Malley，1990；Cato 等，1992）和金属调节元件（Silver 和 Walderhaugh，1992）来说明。在真核 mRNA 中，IRE（铁调节元件）是唯一具有特征的顺式（非编码）调节元件家族（Theil，1990、1993 综述；Klausner 等，1993）。 IRE 似乎是铁特异性调节元件与通用翻译控制元件或 mRNA 稳定性元件融合的复合物（图 1a）。当细胞铁浓度较低时，铁蛋白 mRNA 不会被翻译（Zahringer 等，1976；Schaefer 和 Theil，1981；Shull 和 Theil，1982）。同时，转铁蛋白受体 (TfR) mRNA 得到稳定和翻译（Mullner 和 Kuhn，1988）。因此，当没有铁可供浓缩或储存时，细胞就省去了合成铁蛋白的费用，并且 TfR 合成允许铁的吸收。当铁水平较高时，铁蛋白 mRNA 会被翻译，TfR mRNA 会被降解，从而允许铁储存并阻止铁吸收。铁储存和铁吸收的协调调节取决于蛋白质调节剂、铁调节蛋白 (IRP) 和 IRE mRNA 序列家族的相互作用 (Aziz 和 Munro, 1987; Hentze 等人, 1987a, b; Casey 等人, 1988; Walden 等人, 1989; Rouault 等人, 1990; Theil,(a)
Families of related cis regulatory elements in DNA create a recurring theme that is illustrated by the glucocorticoid receptor elements (eg Diamond et al., 1990; O'Malley, 1990; Cato et al., 1992) and metal regulatory elements (Silver and Walderhaugh, 1992). In eukaryotic mRNAs, IREs (Iron Regulatory Elements) are the only characterized family of cis (non-coding) regulatory elements (reviewed in Theil, 1990, 1993; Klausner et al., 1993). IREs appear to be composites of an iron-specific regulatory element fused to either a generic translation control element or mRNA stability element (Figure la). When cellular iron concentrations are low, ferritin mRNA is not translated (Zahringer et al., 1976; Schaefer and Theil, 1981; Shull and Theil, 1982). At the same time, the transferrin receptor (TfR) mRNA is stabilized and translated (Mullner and Kuhn, 1988). Thus when no iron is available to concentrate or to store, the cell is spared the expense of synthesizing ferritin and TfR synthesis allows iron uptake. When iron levels are high, ferritin mRNA is translated and TfR mRNA is degraded, allowing iron storage and preventing iron uptake. Co-ordinated regulation of iron storage and iron uptake depends on the interaction of a protein regulator, the iron regulatory protein (IRP), and the IRE family of mRNA sequences (Aziz and Munro, 1987; Hentze et al., 1987a, b; Casey et al., 1988; Walden et al., 1989; Rouault et al., 1990; Theil,(a)