Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots
Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots
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DOI：
10.1016/j.femsle.2005.05.006
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发表时间：
2005-06-15
影响因子：
2.1
通讯作者：
Zhou, SN
中科院分区：
文献类型：
--
作者：
Cao, LX;Qiu, ZQ;Zhou, SN
从香蕉根中成功分离到131株内生放线菌。其中链霉菌属（99株）、轮状链霉菌属（28株）和孢链菌属（2株），其余2株未被鉴定。在香蕉组织提取液培养基上，约有18.3%的分离菌株对致病的古巴尖孢镰刀菌生长有抑制作用。最常见的分离菌株S96与灰绿链霉菌相似。约37.5%的灰褐色葡萄球菌菌株对古巴尖孢镰刀菌具有拮抗作用。当FeCl3进入抑制区后，菌株S96的拮抗作用消失。体内生物防治试验结果表明，与未施用菌株相比，经菌株S96处理的青苗的疾病严重程度指数（DSI）显著降低（P = 0.05），平均鲜重显著增加（P = 0.001）。研究结果表明，利用产铁载体内生链霉菌对香蕉枯萎病进行生物防治具有一定的潜力。(c) 2005年欧洲微生物学会联合会。Elsevier B.V.版权所有。
A total of 131 endophytic actinomycete strains were successfully isolated from surface-sterilized banana roots. These isolates belonged to Streptomyces (n = 99), Streptoverticillium (n = 28), and Streptosporangium (n = 2) spp. The remaining 2 isolates were not identified. About 18.3 % of the isolates inhibited the growth of pathogenic Fusarium oxysporum f. sp. cubense on banana tissue extract medium. The most frequently isolated Streptomyces sp. strain S96 was similar to Streptomyces griseorubiginosus. About 37.5 % of the S. griseorubiginosus strains were antagonistic to F oxysporum f. sp. cubense. The antagonism of strain S96 was lost when FeCl3 was introduced into the inhibition zone. In vivo biocontrol assays showed that the disease severity index (DSI) was significantly (P = 0.05) reduced and mean fresh weight increased (P = 0.001) in plantlets treated with strain S96 compared to those grown in the absence of the biocontrol strain. These findings indicate the potential of developing siderophore-producing Streptomyces endophytes for the biological control of fusarium wilt disease of banana. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.