Differential distribution of Fos expression within the male rat preoptic area and hypothalamus in response to physical vs. psychological stress
Differential distribution of Fos expression within the male rat preoptic area and hypothalamus in response to physical vs. psychological stress
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DOI：
10.1016/s0361-9230(01)00532-9
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发表时间：
2001-06-01
影响因子：
3.8
通讯作者：
Gillen, E
中科院分区：
文献类型：
--
作者：
Briski, K;Gillen, E
最近关于压力诱导的肾上腺糖皮质激素分泌的研究表明，个体压力刺激对激素释放的影响在数量上存在差异，这表明下丘脑-垂体-肾上腺轴对此类挑战（尤其是心理压力源和身体压力源）表现出区别性而非普遍的反应。立即早期基因 cfos 由中枢神经系统神经元表达，以响应包括压力在内的多种生理刺激。以下研究调查了视前区和下丘脑中 Fos 免疫标记的分布和/或强度在施加压力后是否有所不同；不同强度的。在（1）转移到新环境（NE应激）、（2）限制在约束管内（休息应激）或（3）固定（IM）应激后2小时，通过经心灌注处死雄性大鼠组。无应激的对照组在其家中的笼子里保持不受干扰。虽然 NE 应激组仅在下丘脑前部和后前部表现出 Fos 免疫反应性 (ir)，但两种物理应激源均诱导外侧视前区、正中视前区、室旁核、弓状核、下丘脑背内侧核和外侧下丘脑中 Fos 的免疫染色，而 IM 应激组中 Fos-ir 阳性神经元的数量通常较多。在 IM 应激大鼠中，在视上核和视交叉上核中观察到额外的 Fos-ir。这些研究表明，响应新环境相对温和的应激而表达 Fos-ir 的神经元的分布与那些由更令人厌恶的应激源转录激活的神经元的分布不同。研究发现，在暴露于休息或 IM 压力后，Fos-ir 出现在几个常见部位，这表明局部神经元群可能包含由强烈压力激活的常见神经通路。 (C) 2001 爱思唯尔科学公司。
Recent studies on stress-induced adrenal glucocorticoid secretion have demonstrated quantitatively different effects of individual stress stimuli on hormone release, suggesting that the hypothalamic-pituitary-adrenal axis exhibits discriminative, rather than ubiquitous responses to such challenges, particularly psychological vs. physical stressors. The immediate-early gene, cfos, is expressed by central nervous system neurons in response to numerous physiological stimuli, including stress. The following study investigated whether the distribution and/or intensity of immunolabeling for Fos in the preoptic area and hypothalamus differ after imposition of stressors; of variable intensity. Groups of male rats were sacrificed by transcardial perfusion 2 h after (1) transfer to a novel environment (NE stress), (2) confinement within a restraint tube (REST stress), or (3) immobilization (IM) stress. Nonstressed controls remained undisturbed in their home cages. Whereas the NE-stress group exhibited Fos immunoreactivity (ir) only within anterior and lateroanterior hypothalamus, both physical stressors induced immunostaining for Fos in the lateral preoptic area, median preoptic, paraventricular, arcuate, dorsomedial hypothalamic nuclei, and lateral hypothalamus, while numbers of Fos-ir-positive neurons were generally greater in the IM vs. REST stress group. In the IM-stressed rats, additional Fos-ir was observed in the supraoptic and suprachiasmatic nuclei. These studies show that neurons expressing Fos-ir in response to the relatively mild stress of novel environment are distributed differently than those that are transcriptionally activated by more aversive stressors. Findings that Fos-ir occurs in several common sites following exposure to REST or IM stress suggest that local neuron populations may comprise a common neural pathway(s) that is activated by intense forms of stress. (C) 2001 Elsevier Science Inc.