Lessons learned from quantitative fundus autofluorescence.
Lessons learned from quantitative fundus autofluorescence.
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DOI：
10.1016/j.preteyeres.2019.100774
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发表时间：
2020-01
影响因子：
17.8
通讯作者：
de Carvalho JRL Jr
中科院分区：
文献类型：
--
作者：
Sparrow JR;Duncker T;Schuerch K;Paavo M;de Carvalho JRL Jr
定量眼底自发荧光（qAF）是建立在共焦扫描激光平台上的一种方法，用于测量由短波长（488 nm）激发引起的视网膜固有自发荧光的强度。由于是非侵入性的，qAF不会中断组织结构，因此允许结构相关性。眼底自然自发荧光的光谱特征、细胞起源和地形分布表明，它是从视网膜色素加合物发出的，视网膜色素加合物在感光细胞中形成，在大多数情况下在视网膜色素上皮细胞中积累。在许多视网膜疾病中，眼底自发荧光的分布和强度偏离正常，并且广泛认识到这些变化的模式可以帮助视网膜疾病的诊断和监测。qAF采用的标准化协议涉及将眼底灰度级标准化为安装在成像仪器中的荧光参考。与放大率和前介质吸收校正一起，该方法便于与系列图像和患者组内采集的图像进行比较。在这里，我们提供了一个全面的总结qAF的原则和实践，我们强调最近的努力，阐明视网膜疾病的过程相结合的qAF与多模式成像。
Quantitative fundus autofluorescence (qAF) is an approach that is built on a confocal scanning laser platform and used to measure the intensity of the inherent autofluorescence of retina elicited by short-wavelength (488 nm) excitation. Being non-invasive, qAF does not interrupt tissue architecture, thus allowing for structural correlations. The spectral features, cellular origin and topographic distribution of the natural autofluorescence of the fundus indicate that it is emitted from retinaldehyde-adducts that form in photoreceptor cells and accumulate, under most conditions, in retinal pigment epithelial cells. The distributions and intensities of fundus autofluorescence deviate from normal in many retinal disorders and it is widely recognized that these changing patterns can aid in the diagnosis and monitoring of retinal disease. The standardized protocol employed by qAF involves the normalization of fundus grey levels to a fluorescent reference installed in the imaging instrument. Together with corrections for magnification and anterior media absorption, this approach facilitates comparisons with serial images and images acquired within groups of patients. Here we provide a comprehensive summary of the principles and practice of qAF and we highlight recent efforts to elucidate retinal disease processes by combining qAF with multi-modal imaging.
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