Adenovirus type 11 uses CD46 as a cellular receptor
Adenovirus type 11 uses CD46 as a cellular receptor
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DOI：
10.1128/jvi.77.17.9183-9191.2003
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发表时间：
2003-09-01
影响因子：
5.4
通讯作者：
Arnberg, N
中科院分区：
文献类型：
--
作者：
Segerman, A;Atkinson, JP;Arnberg, N
51种人类腺病毒血清型分为6种（A至F）。在体外，许多腺病毒使用柯萨奇-腺病毒受体（CAR）附着在宿主细胞上。B种腺病毒不与CAR结合血清型竞争与宿主细胞的结合，有人认为B种腺病毒使用CAR以外的受体。B种腺病毒主要引起呼吸道、眼睛和泌尿道的疾病。在这里，我们证明了11型腺病毒（Ad11； B种）与转染CD46（膜辅助因子蛋白）-cDNA的中国鼠卵巢（CHO）细胞的结合强度至少是转染编码CAR或CD55（衰变加速因子）的cdna的CHO细胞的10倍。在转染CD46-cDNA后，非允许型CHO细胞对Ad11具有允许性。可溶性Ad11纤维旋钮抑制Ad11病毒粒子与转染cd46细胞的结合，而Ad7和Ad5旋钮不抑制Ad11病毒粒子与转染cd46细胞的结合，抗cd46抗体抑制Ad11的结合和感染。从这些结果我们得出结论，CD46是Ad11的细胞受体。
The 51 human adenovirus serotypes are divided into six species (A to F). Many adenoviruses use the coxsackie-adenovirus receptor (CAR) for attachment to host cells in vitro. Species B adenoviruses do not compete with CAR-binding serotypes for binding to host cells, and it has been suggested that species B adenoviruses use a receptor other than CAR. Species B adenoviruses mainly cause disease in the respiratory tract, the eyes, and in the urinary tract. Here we demonstrate that adenovirus type 11 (Ad11; of species B) binds to Chinese hamster ovary (CHO) cells transfected with CD46 (membrane cofactor protein)-cDNA at least 10 times more strongly than to CHO cells transfected with cDNAs encoding CAR or CD55 (decay accelerating factor). Nonpermissive CHO cells were rendered permissive to Ad11 infection upon transfection with CD46-cDNA. Soluble Ad11 fiber knob but not Ad7 or Ad5 knob inhibited binding of Ad11 virions to CD46-transfected cells, and anti-CD46 antibodies inhibited both binding of and infection by Ad11. From these results we conclude that CD46 is a cellular receptor for Ad11.