Use of Flow Cytometry To Monitor Legionella Viability
Use of Flow Cytometry To Monitor Legionella Viability
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DOI：
10.1128/aem.01364-08
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Riffard, Serge
中科院分区：
文献类型：
--
作者：
Allegra, Severine;Berger, Francoise;Riffard, Serge
在70℃热休克处理期间，用流式细胞术（FCA）监测军团菌活力。处理30分钟后，在12个菌株中有6个菌株，FCA仍然检测到10 - 25%的活细胞但不可培养（VBNC）。这些VBNC细胞能够产生ATP，并在变形虫上培养后复苏。
Legionella viability was monitored during heat shock treatment at 70 C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae.