Isolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia
Isolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia
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DOI：
10.5694/mja2.50569
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发表时间：
2020-04-01
影响因子：
11.4
通讯作者：
Catton, Mike G.
中科院分区：
文献类型：
--
作者：
Caly, Leon;Druce, Julian;Catton, Mike G.
目的描述SARS-CoV-2在澳大利亚的首次分离和测序以及该分离株的快速共享。背景SARS-CoV-2是从一名来自中国武汉的58岁男子中分离出来的，该男子于2020年1月19日抵达墨尔本，并于2020年1月24日从急诊科入住墨尔本莫纳什医疗中心，主要结果首例COVID-19报告病例的临床病程和实验室特征（SARS-CoV-2引起的疾病）在澳大利亚;分离，全基因组测序，成像，结果患者入院时采集的鼻咽拭子和痰液均为SARS阳性，CoV-2（逆转录聚合酶链反应）。用来自鼻咽拭子的材料接种Vero/hSLAM细胞导致在培养物中分离SARS-CoV-2病毒。上清液的电子显微镜检查证实存在具有冠状病毒科病毒形态特征的病毒颗粒。全基因组测序和系统发育分析表明，该病毒分离株与其他公开的SARS-CoV-2基因组具有大于99.99%的序列同源性。在24小时的隔离，第一个澳大利亚SARS-CoV-2分离共享与当地和海外的参考实验室和主要的北美和欧洲culturecollection.Conclusions的能力，迅速识别，传播，并在国际上分享我们的SARS-CoV-2分离是一个重要的一步，在合作的科学努力，以有效地处理这一国际公共卫生紧急情况，通过开发更好的诊断程序，候选疫苗和抗病毒剂。
Objectives To describe the first isolation and sequencing of SARS-CoV-2 in Australia and rapid sharing of the isolate.Setting SARS-CoV-2 was isolated from a 58-year-old man from Wuhan, China who arrived in Melbourne on 19 January 2020 and was admitted to the Monash Medical Centre, Melbourne from the emergency department on 24 January 2020 with fever, cough, and progressive dyspnoea.Major outcomes Clinical course and laboratory features of the first reported case of COVID-19 (the illness caused by SARS-CoV-2) in Australia; isolation, whole genome sequencing, imaging, and rapid sharing of virus from the patient.Results A nasopharyngeal swab and sputum collected when the patient presented to hospital were each positive for SARS-CoV-2 (reverse transcription polymerase chain reaction). Inoculation of Vero/hSLAM cells with material from the nasopharyngeal swab led to the isolation of SARS-CoV-2 virus in culture. Electron microscopy of the supernatant confirmed the presence of virus particles with morphology characteristic of viruses of the family Coronaviridae. Whole genome sequencing of the viral isolate and phylogenetic analysis indicated the isolate exhibited greater than 99.99% sequence identity with other publicly available SARS-CoV-2 genomes. Within 24 hours of isolation, the first Australian SARS-CoV-2 isolate was shared with local and overseas reference laboratories and major North American and European culture collections.Conclusions The ability to rapidly identify, propagate, and internationally share our SARS-CoV-2 isolate is an important step in collaborative scientific efforts to deal effectively with this international public health emergency by developing better diagnostic procedures, vaccine candidates, and antiviral agents.