FORMATION OF 8-HYDROXYGUANINE MOIETY IN CELLULAR DNA BY AGENTS PRODUCING OXYGEN RADICALS AND EVIDENCE FOR ITS REPAIR
FORMATION OF 8-HYDROXYGUANINE MOIETY IN CELLULAR DNA BY AGENTS PRODUCING OXYGEN RADICALS AND EVIDENCE FOR ITS REPAIR
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DOI：
10.1093/carcin/7.11.1849
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发表时间：
1986-11-01
期刊：
影响因子：
4.7
通讯作者：
TANOOKA, H
中科院分区：
文献类型：
--
作者：
KASAI, H;CRAIN, PF;TANOOKA, H
8-在用X射线照射组织培养物中的细胞后，从HeLa细胞分离的DNA中检测到羟基脱氧鸟苷（8-OH-dG），并且在用γ射线照射整个动物后，从小鼠肝脏分离的DNA中检测到羟基脱氧鸟苷（8-OH-dG）。射线体内照射后DNA中8-OH-dG的含量比体外照射后低3个数量级（0.008-0.032 8-OH-dG残基/105 dG/krad）。通过照射小鼠在肝DNA中产生的8-OH-dG随时间减少，表明在小鼠肝中存在作用于8-OH-dG的修复酶。用过氧化氢处理鼠伤寒沙门氏菌细胞也引起8-OH-dG含量的增加。这些结果表明，8-OH-dG是在体内形成的细胞DNA处理与各种氧自由基产生剂，它是可修复的。
8-Hydroxydeoxyguanosine (8-OH-dG) was detected in DNA isolated from HeLa cells after the cells in tissue culture had been irradiated with X-rays and from the liver of mice after the whole animals had been irradiated with .gamma.-rays. The amounts of 8-OH-dG in DNA after in vivo irradiation were three orders of magnitude lower than those after in vitro irradiation (0.008-0.032 8-OH-dG residue/105 dG/krad). The 8-OH-dG produced in liver DNA by irradiation of mice decreased with time, suggesting the presence of a repair enzyme(s) acting on 8-OH-dG in mouse liver. Treatment of Salmonella typhimurium cells with hydrogen peroxide also caused increase in the 8-OH-dG content. These results indicate that 8-OH-dG is formed in vivo in cellular DNA on treatment with various oxygen radical-producing agents and that it is repairable.