Genetic Variation among Staphylococcus aureus Strains from Bovine Milk and Their Relevance to Methicillin-Resistant Isolates from Humans
Genetic Variation among Staphylococcus aureus Strains from Bovine Milk and Their Relevance to Methicillin-Resistant Isolates from Humans
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DOI：
10.1128/jcm.01940-09
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发表时间：
2010-06-01
影响因子：
9.4
通讯作者：
Eguchi, Masashi
中科院分区：
文献类型：
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作者：
Hata, Eiji;Katsuda, Ken;Eguchi, Masashi
在牛乳腺炎重要病原菌金黄色葡萄球菌分离株的遗传分析中，多位点序列分型（MLST）与脉冲场凝胶电泳、coa pcr -限制性片段长度多态性（RFLP）、spa分型和凝固酶血清分型结果具有较强的相关性。根据MLST结果，序列97型（ST97）和ST705菌株不仅是日本牛金黄色葡萄球菌的优势菌株，而且可能是牛金黄色葡萄球菌的流行菌株。虽然从系统发育分析来看，这两个谱系似乎是远亲，但它们都具有共同的特征，即lukM/lukF'-PV和凝固酶血清型VI，这些特征在牛和人的小菌株中非常罕见，可能有助于这些谱系的宿主特异性。日本首次从牛乳中发现4株耐甲氧西林金黄色葡萄球菌（MRSA）；这些分离株的基因型和血清型与日本的人MRSA分离株相同或相似（ST5，葡萄球菌盒染色体mec II型[SCCmec II]， Spa型t002或t375，凝固酶血清型II， ST89, SCCmec IIIa， Spa型t5266，凝固酶血清型I）。ST5和ST89在世界牛分离株中并不常见，而这些STs在日本的人类MRSA分离株中很常见。
In genetic analysis of bovine Staphylococcus aureus isolates that are recognized as an important pathogenic bacterium in bovine mastitis, multilocus sequence typing (MLST) showed strong correlation to the results of pulsed-field gel electrophoresis, coa PCR-restriction fragment length polymorphism (RFLP), spa typing, and the coagulase serotyping method. According to MLST results, strains derived from sequence type 97 (ST97) and ST705 were suggested as not only dominant bovine S. aureus lineages in Japan but also pandemic bovine S. aureus lineages. Although both lineages seem to be distantly related to each other by phylogenetic analysis, both had common characteristics, i.e., lukM/lukF'-PV and coagulase serotype VI. These characteristics were very rare among minor bovine strains and human strains and may contribute to the host specificity of these lineages. Four methicillin-resistant S. aureus (MRSA) isolates were first confirmed from bovine milk in Japan; these isolates showed geno-and serotypes that were identical or similar to those of human MRSA isolates in Japan (ST5, staphylococcal cassette chromosome mec type II [SCCmec II], Spa type t002 or t375, and coagulase serotype II, and ST89, SCCmec IIIa, Spa type t5266, and coagulase serotype I). ST5 and ST89 are uncommon among bovine isolates in the world, whereas these STs are common among human MRSA isolates in Japan.