High levels of adenovirus DNA in serum correlate with fatal outcome of adenovirus infection in children after allogeneic stem-cell transplantation
High levels of adenovirus DNA in serum correlate with fatal outcome of adenovirus infection in children after allogeneic stem-cell transplantation
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DOI：
10.1086/341770
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发表时间：
2002-09-01
影响因子：
11.8
通讯作者：
van Tol, MJ
中科院分区：
文献类型：
--
作者：
Schilham, MW;Claas, EC;van Tol, MJ
腺病毒（AdV）感染的发病率增加，导致死亡的儿童谁经历了异基因干细胞移植，有必要找到新的方法来监测AdV感染。在这项回顾性研究中，从36个移植受者粪便培养阳性腺病毒的血清样本中的腺病毒DNA水平进行了测定聚合酶链反应（PCR）半定量分析系列稀释的DNA模板。7例死于AdV感染的儿童中有6例（86%）血清AdV DNA水平高（PCR检测，DNA模板稀释度大于或等于100倍; P
An increase in the incidence of adenovirus (AdV) infection leading to death among children who have undergone allogeneic stem-cell transplantation has made it necessary to find new ways to monitor AdV infection. In this retrospective study, levels of AdV DNA in serum samples obtained from 36 transplant recipients with stool cultures positive for AdV were measured by polymerase chain reaction (PCR) semiquantitatively by analyzing serial dilutions of the DNA template. Six (86%) of 7 children who died of AdV infection, compared with only 2 (7%) of 29 other patients, had high serum levels of AdV DNA (detectable by PCR at a greater than or equal to100-fold dilution of the DNA template; P