High Salt Diet Affects Renal Sodium Excretion and ERRα Expression.
High Salt Diet Affects Renal Sodium Excretion and ERRα Expression.
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高盐饮食影响肾钠排泄和 ERR α 表达
DOI：
10.3390/ijms17040480
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发表时间：
2016-04-01
影响因子：
5.6
通讯作者：
Mu JJ
中科院分区：
文献类型：
--
作者：
Wang D;Wang Y;Liu FQ;Yuan ZY;Mu JJ
肾脏调节水和钠的平衡，因此与血压有关。雌激素相关受体α（ERRα）是一种在肾脏中高度表达的孤儿核受体和转录因子，目前尚不清楚它是否影响水和钠的重吸收。本研究旨在探讨ERRα、Na+/K+-ATP酶和上皮钠通道（ENaC）蛋白表达的变化是否影响Dahl盐敏感（DS）大鼠肾脏水钠重吸收。与DS大鼠同源性为98%的SS.13BN大鼠用作正常血压对照组。6周高盐饮食干预后，DS和SS.13BN大鼠的24 h尿钠排泄量增加，而大豆苷元（ERRα激动剂）治疗的DS大鼠的24 h尿钠排泄量增加。高钠饮食处理后，DS大鼠ERRα表达降低，β-和γ-ENaC mRNA表达增加。在染色质免疫沉淀（CHIP）试验中，在用抗ERR α抗体处理的样品中获得阳性PCR信号。高盐饮食干预后ERRα转录活性降低。ERRα通过与ENaC启动子结合，降低β-和γ-ENaC的表达，从而增加Na+重吸收。因此，ERRα可能是盐敏感性高血压的发病因素之一。
Kidneys regulate the balance of water and sodium and therefore are related to blood pressure. It is unclear whether estrogen-related receptor α (ERRα), an orphan nuclear receptor and transcription factor highly expressed in kidneys, affects the reabsorption of water and sodium. The aim of this study was to determine whether changes in the expressions of ERRα, Na+/K+-ATPase and epithelial sodium channel (ENaC) proteins affected the reabsorption of water and sodium in kidneys of Dahl salt-sensitive (DS) rats. SS.13BN rats, 98% homologous to the DS rats, were used as a normotensive control group. The 24 h urinary sodium excretion of the DS and SS.13BN rats increased after the 6-week high salt diet intervention, while sodium excretion was increased in DS rats with daidzein (agonist of ERRα) treatment. ERRα expression was decreased, while β- and γ-ENaC mRNA expressions were increased upon high sodium diet treatment in the DS rats. In the chromatin immunoprecipitation (CHIP) assay, positive PCR signals were obtained in samples treated with anti-ERRα antibody. The transcriptional activity of ERRα was decreased upon high salt diet intervention. ERRα reduced the expressions of β- and γ-ENaC by binding to the ENaC promoter, thereby increased Na+ reabsorption. Therefore, ERRα might be one of the factors causing salt-sensitive hypertension.